ETS1 Antibody - #DF6488

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产品: ETS1 抗体
货号: DF6488
描述: Rabbit polyclonal antibody to ETS1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 50kDa; 50kD(Calculated).
蛋白号: P14921
RRID: AB_2838450

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
ETS1 Antibody detects endogenous levels of total ETS1.
RRID:
AB_2838450
引用格式: Affinity Biosciences Cat# DF6488, RRID:AB_2838450.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Avian erythroblastosis virus E26 (v ets) oncogene homolog 1; C ets 1 protein; c-ets-1; ETS 1; Ets protein; ETS proto-oncogene 1, transcription factor; ETS1; ETS1 oncogene; ETS1 protein; ETS1_HUMAN; EWSR 2; EWSR2; FLJ10768; Oncogene ETS1; P54; Protein C-ets-1; v ets avian erythroblastosis virus E2 oncogene homolog; v ets avian erythroblastosis virus E2 oncogene homolog 1; v ets avian erythroblastosis virus E26 oncogene homolog 1; v ets erythroblastosis virus E26 oncogene homolog 1; v-ets erythroblastosis virus E26 oncogene homolog 1;

抗原和靶标

免疫原:
Uniprot:

P14921(ETS1_HUMAN) >>Visit HPA database.

基因/基因ID:
ETS1(2113)
表达:
P14921 ETS1_HUMAN:

Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.

描述:
This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
序列:
MKAAVDLKPTLTIIKTEKVDLELFPSPDMECADVPLLTPSSKEMMSQALKATFSGFTKEQQRLGIPKDPRQWTETHVRDWVMWAVNEFSLKGVDFQKFCMNGAALCALGKDCFLELAPDFVGDILWEHLEILQKEDVKPYQVNGVNPAYPESRYTSDYFISYGIEHAQCVPPSEFSEPSFITESYQTLHPISSEELLSLKYENDYPSVILRDPLQTDTLQNDYFAIKQEVVTPDNMCMGRTSRGKLGGQDSFESIESYDSCDRLTQSWSSQSSFNSLQRVPSYDSFDSEDYPAALPNHKPKGTFKDYVRDRADLNKDKPVIPAAALAGYTGSGPIQLWQFLLELLTDKSCQSFISWTGDGWEFKLSDPDEVARRWGKRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQSLLGYTPEELHAMLDVKPDADE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P14921 作为底物

Site PTM Type Enzyme
K2 Acetylation
K2 Ubiquitination
K8 Acetylation
K8 Ubiquitination
K15 Acetylation
K15 Sumoylation
K15 Ubiquitination
K18 Ubiquitination
S26 Phosphorylation
T38 Phosphorylation P28482 (MAPK1) , Q13164 (MAPK7) , P27361 (MAPK3)
S41 Phosphorylation
K42 Ubiquitination
S46 Phosphorylation
K50 Ubiquitination
S54 Phosphorylation
K58 Ubiquitination
K67 Ubiquitination
K97 Ubiquitination
K138 Sumoylation
K138 Ubiquitination
Y140 Phosphorylation
Y158 Phosphorylation
K200 Sumoylation
Y205 Phosphorylation
S207 Phosphorylation
Y223 Phosphorylation
K227 Ubiquitination
T232 Phosphorylation
K245 Acetylation
K245 Ubiquitination
S251 Phosphorylation Q9UQM7 (CAMK2A)
S254 Phosphorylation
S257 Phosphorylation Q9UQM7 (CAMK2A)
Y258 Phosphorylation
S260 Phosphorylation
T265 Phosphorylation
S267 Phosphorylation
S270 Phosphorylation
S272 Phosphorylation
S273 Phosphorylation
S276 Phosphorylation
S282 Phosphorylation Q9UQM7 (CAMK2A)
Y283 Phosphorylation
S285 Phosphorylation Q9UQM7 (CAMK2A)
S288 Phosphorylation
Y291 Phosphorylation
K299 Ubiquitination
K301 Ubiquitination
K305 Acetylation
K305 Ubiquitination
K377 Ubiquitination
K383 Ubiquitination
K388 Sumoylation
K388 Ubiquitination
Y395 Phosphorylation
K399 Ubiquitination
K404 Ubiquitination
T405 Phosphorylation
Y410 Phosphorylation

研究背景

功能:

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion.

翻译修饰:

Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.

Ubiquitinated; which induces proteasomal degradation.

细胞定位:

Cytoplasm. Nucleus.
Note: Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed within lymphoid cells. Isoforms c-ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.

亚基结构:

Binds DNA as a homodimer; homodimerization is required for transcription activation. Interacts with MAF and MAFB. Interacts with PAX5; the interaction alters DNA-binding properties (By similarity). Interacts with DAXX. Interacts with UBE2I. Interacts with SP100; the interaction is direct and modulates ETS1 transcriptional activity.

蛋白家族:

Belongs to the ETS family.

研究领域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

文献引用

1). In Vivo Inhibition of MicroRNA-326 in a NOD. H-2h4 Mouse Model of Autoimmune Thyroiditis. Frontiers in Immunology, 2021 (PubMed: 34140947) [IF=7.3]

Application: IF/ICC    Species: Mouse    Sample: thyroid

FIGURE 3 | Ets-1 protein expression was increased in the spleens of NOD.H-2h4 mice in the LV-sponge groups.(C) Immunofluorescence staining of CD4+ Ets-1+ cells in thyroid sections. DAPI, blue; CD4+ Ets-1+ cells are shown in cyan in the nucleus (CD4+, red; Ets-1+, green). Under high-power (HP)microscope, three view fields of thyroid section were randomly selected to count the number of CD4+Ets-1+ cells. Scale bar, 100 mm.

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