Syndecan 4 Antibody - #AF0831

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产品: Syndecan 4 抗体
货号: AF0831
描述: Rabbit polyclonal antibody to Syndecan 4
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 22kDa; 22kD(Calculated).
蛋白号: P31431
RRID: AB_2833791

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(86%), Chicken(100%)
克隆:
Polyclonal
特异性:
Syndecan 4 Antibody detects endogenous levels of total Syndecan 4.
RRID:
AB_2833791
引用格式: Affinity Biosciences Cat# AF0831, RRID:AB_2833791.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4;

抗原和靶标

免疫原:
Uniprot:

P31431(SDC4_HUMAN) >>Visit HPA database.

基因/基因ID:
SDC4(6385)
表达:
P31431 SDC4_HUMAN:

Expressed in epithelial and fibroblastic cells.

描述:
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. SwissProt Information
序列:
MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Dog
86
Rabbit
86
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P31431 作为底物

Site PTM Type Enzyme
S97 O-Glycosylation
T101 O-Glycosylation
K104 Ubiquitination
S117 O-Glycosylation
S126 O-Glycosylation
T134 Phosphorylation
K174 Ubiquitination
S179 Phosphorylation Q05655 (PRKCD)
Y180 Phosphorylation P12931 (SRC)
K190 Ubiquitination
T193 Phosphorylation
Y197 Phosphorylation

研究背景

功能:

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

翻译修饰:

Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

细胞定位:

Membrane>Single-pass type I membrane protein. Secreted.
Note: Shedding of the ectodomain produces a soluble form.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in epithelial and fibroblastic cells.

亚基结构:

Homodimer. Interacts (via its cytoplasmic domain) with GIPC (via its PDZ domain). Interacts (via its cytoplasmic domain) with NUDT16L1 (By similarity). Interacts with CDCP1 and SDCBP.

蛋白家族:

Belongs to the syndecan proteoglycan family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

文献引用

1). Necroptotic TNFα‐syndecan 4‐TNFα vicious cycle as a therapeutic target for preventing temporomandibular joint osteoarthritis. Journal of Bone and Mineral Research (PubMed: 35278225) [IF=6.2]

Application: IHC    Species: Rat    Sample:

Fig. 5 SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by interference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were measured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data of C (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of cartilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data between two groups.

Application: WB    Species: Rat    Sample:

Fig. 5 SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by interference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were measured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data of C (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of cartilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data between two groups.

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