TLR5 Antibody - #DF6575

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产品: TLR5 抗体
货号: DF6575
描述: Rabbit polyclonal antibody to TLR5
应用: WB IHC
反应: Human, Mouse, Rat
预测: Chicken, Xenopus
分子量: 98kDa; 98kD(Calculated).
蛋白号: O60602
RRID: AB_2838537

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
TLR5 Antibody detects endogenous levels of total TLR5.
RRID:
AB_2838537
引用格式: Affinity Biosciences Cat# DF6575, RRID:AB_2838537.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ10052; MGC126430; MGC126431; SLEB1; TIL 3; TIL3; TLR 5; Tlr5; TLR5_HUMAN; Toll like receptor 5; Toll like receptor 5 precursor; Toll-like receptor 5; Toll/interleukin 1 receptor like protein 3; Toll/interleukin-1 receptor-like protein 3;

抗原和靶标

免疫原:
Uniprot:

O60602(TLR5_HUMAN) >>Visit HPA database.

基因/基因ID:
TLR5(7100)
表达:
O60602 TLR5_HUMAN:

Highly expressed on the basolateral surface of intestinal epithelia (PubMed:11489966). Expressed also in other cells such as lung epithelial cells (PubMed:11489966, PubMed:18490781).

描述:
This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]
序列:
MGDHLDLLLGVVLMAGPVFGIPSCSFDGRIAFYRFCNLTQVPQVLNTTERLLLSFNYIRTVTASSFPFLEQLQLLELGSQYTPLTIDKEAFRNLPNLRILDLGSSKIYFLHPDAFQGLFHLFELRLYFCGLSDAVLKDGYFRNLKALTRLDLSKNQIRSLYLHPSFGKLNSLKSIDFSSNQIFLVCEHELEPLQGKTLSFFSLAANSLYSRVSVDWGKCMNPFRNMVLEILDVSGNGWTVDITGNFSNAISKSQAFSLILAHHIMGAGFGFHNIKDPDQNTFAGLARSSVRHLDLSHGFVFSLNSRVFETLKDLKVLNLAYNKINKIADEAFYGLDNLQVLNLSYNLLGELYSSNFYGLPKVAYIDLQKNHIAIIQDQTFKFLEKLQTLDLRDNALTTIHFIPSIPDIFLSGNKLVTLPKINLTANLIHLSENRLENLDILYFLLRVPHLQILILNQNRFSSCSGDQTPSENPSLEQLFLGENMLQLAWETELCWDVFEGLSHLQVLYLNHNYLNSLPPGVFSHLTALRGLSLNSNRLTVLSHNDLPANLEILDISRNQLLAPNPDVFVSLSVLDITHNKFICECELSTFINWLNHTNVTIAGPPADIYCVYPDSFSGVSLFSLSTEGCDEEEVLKSLKFSLFIVCTVTLTLFLMTILTVTKFRGFCFICYKTAQRLVFKDHPQGTEPDMYKYDAYLCFSSKDFTWVQNALLKHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLEAFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDFQDVGWFLHKLSQQILKKEKEKKKDNNIPLQTVATIS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Xenopus
100
Chicken
100
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60602 作为底物

Site PTM Type Enzyme
N37 N-Glycosylation
N46 N-Glycosylation
Y108 Phosphorylation
Y161 Phosphorylation
N245 N-Glycosylation
N342 N-Glycosylation
N422 N-Glycosylation
N595 N-Glycosylation
N598 N-Glycosylation
K680 Acetylation
Y798 Phosphorylation
S805 Phosphorylation Q15139 (PRKD1)

研究背景

功能:

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).

翻译修饰:

Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines.

Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed on the basolateral surface of intestinal epithelia. Expressed also in other cells such as lung epithelial cells.

亚基结构:

Homodimer. Interacts with MYD88 (via TIR domain). Interacts with TICAM1 (via TIR domain). Interacts with UNC93B1; this interaction is essential for proper TLR5 localization to the plasma membrane.

蛋白家族:

Belongs to the Toll-like receptor family.

研究领域

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Toll-like receptor signaling pathway.   (View pathway)

文献引用

1). 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4. American Journal of Cancer Research (PubMed: 31720079) [IF=5.3]

Application: WB    Species: Human    Sample: HepG2 cells

Figure 5 The migration of HepG2 cells induced by 25-HC is partly mediated by the up-regulation of TLR4. (A) HepG2 cells were treated with the indicated concentrations of 25-HC for 24 hours before mRNA was extracted and expressions of TLR2, TLR4, TLR5, TLR7 and TLR9 were examined by RT-qPCR. (B) HepG2 cells were treated with the indicated concentrations of 25-HC for 24 hours or treated with 25-HC at 10 μM for the indicated times before protein was collected to detect the TLR2, TLR4, TLR5, TLR7 and TLR9 expressions by Western blotting. HepG2 cells were transfected with siNC or siTLR4 or siTLR9 for 24 hours, (C) then cells were treated with the indicated concentrations of 25-HC for 24 hours before proteins were collected and the expressions of MMP1, MMP2, MMP3 and MMP9 were determined by Western blotting. (D) Or migratory ability of HepG2 cells after treated with 25-HC for 36 hours was determined by Transwell assay. Results were obtained from 3 independent experiments and are expressed as the means ± SEM. Statistical significance was determined by Student’s t-test. *P<0.05, ***P<0.001.

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