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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6610, RRID:AB_2838572.
展开/折叠
CD224; D22S672; D22S732; Gamma glutamyl transpeptidase; Gamma glutamyltransferase 1; Gamma glutamyltranspeptidase 1; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1 light chain; GGT 1; GGT; GGT1; GGT1_HUMAN; Glutamyl transpeptidase; Glutathione hydrolase 1; GTG; Leukotriene C4 hydrolase; MGC96892; MGC96904; MGC96963; OTTHUMP00000028921; OTTHUMP00000197959;
抗原和靶标
Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.
- P19440 GGT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKKKLVVLGLLAVVLVLVIVGLCLWLPSASKEPDNHVYTRAAVAADAKQCSKIGRDALRDGGSAVDAAIAALLCVGLMNAHSMGIGGGLFLTIYNSTTRKAEVINAREVAPRLAFATMFNSSEQSQKGGLSVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARQGFPVGKGLAAALENKRTVIEQQPVLCEVFCRDRKVLREGERLTLPQLADTYETLAIEGAQAFYNGSLTAQIVKDIQAAGGIVTAEDLNNYRAELIEHPLNISLGDVVLYMPSAPLSGPVLALILNILKGYNFSRESVESPEQKGLTYHRIVEAFRFAYAKRTLLGDPKFVDVTEVVRNMTSEFFAAQLRAQISDDTTHPISYYKPEFYTPDDGGTAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVGAAGGTQITTATALAIIYNLWFGYDVKRAVEEPRLHNQLLPNVTTVERNIDQAVTAALETRHHHTQIASTFIAVVQAIVRTAGGWAAASDSRKGGEPAGY
翻译修饰 - P19440 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N95 | N-Glycosylation | Uniprot | |
| N120 | N-Glycosylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| N230 | N-Glycosylation | Uniprot | |
| N266 | N-Glycosylation | Uniprot | |
| N297 | N-Glycosylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| N344 | N-Glycosylation | Uniprot | |
| N511 | N-Glycosylation | Uniprot | |
| T550 | Phosphorylation | Uniprot |
研究背景
Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive.
N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity.
Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme.
Cell membrane>Single-pass type II membrane protein.
Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.
Heterodimer composed of the light and heavy chains. The active site is located in the light chain.
Belongs to the gamma-glutamyltransferase family.
研究领域
· Metabolism > Metabolism of other amino acids > Taurine and hypotaurine metabolism.
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
· Metabolism > Lipid metabolism > Arachidonic acid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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