LIG1 Antibody - #DF6663

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产品: LIG1 抗体
货号: DF6663
描述: Rabbit polyclonal antibody to LIG1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 102kDa; 102kD(Calculated).
蛋白号: P18858
RRID: AB_2838625

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(89%), Zebrafish(89%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
LIG1 Antibody detects endogenous levels of total LIG1.
RRID:
AB_2838625
引用格式: Affinity Biosciences Cat# DF6663, RRID:AB_2838625.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1;

抗原和靶标

免疫原:
Uniprot:

P18858(DNLI1_HUMAN) >>Visit HPA database.

基因/基因ID:
LIG1(3978)
描述:
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq, Jul 2008]
序列:
MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQSEDEDREAKRKKEEEEEETPKESLTEAEVATEKEGEDGDQPTTPPKPLKTSKAETPTESVSEPEVATKQELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
89
Bovine
89
Sheep
89
Zebrafish
89
Xenopus
78
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P18858 作为底物

Site PTM Type Enzyme
S4 Phosphorylation
K40 Ubiquitination
S47 Phosphorylation
S49 Phosphorylation
S51 Phosphorylation P24941 (CDK2)
S66 Phosphorylation P68400 (CSNK2A1)
S76 Phosphorylation P06493 (CDK1) , O14757 (CHEK1) , P24941 (CDK2)
K79 Ubiquitination
S88 Phosphorylation
S91 Phosphorylation P24941 (CDK2)
T97 Phosphorylation
S98 Phosphorylation
S109 Phosphorylation
S114 Phosphorylation
S141 Phosphorylation
R146 Methylation
S163 Phosphorylation
T165 Phosphorylation
T171 Phosphorylation
T182 Phosphorylation
T183 Phosphorylation
T190 Phosphorylation
K192 Ubiquitination
T195 Phosphorylation P24941 (CDK2)
T197 Phosphorylation P24941 (CDK2)
S199 Phosphorylation
S201 Phosphorylation
T207 Phosphorylation
K219 Acetylation
K219 Ubiquitination
K226 Ubiquitination
S229 Phosphorylation
S230 Phosphorylation
T233 Phosphorylation P24941 (CDK2)
K244 Sumoylation
K244 Ubiquitination
K270 Ubiquitination
K282 Ubiquitination
K286 Ubiquitination
K298 Ubiquitination
T311 Phosphorylation
S313 Phosphorylation
K376 Ubiquitination
K407 Ubiquitination
K422 Ubiquitination
K428 Ubiquitination
S437 Phosphorylation
S445 Phosphorylation
S447 Phosphorylation
K493 Ubiquitination
T506 Phosphorylation
K533 Ubiquitination
S535 Phosphorylation
K541 Ubiquitination
T548 Phosphorylation
S552 Phosphorylation
K556 Ubiquitination
K568 Ubiquitination
K585 Ubiquitination
K597 Ubiquitination
K630 Sumoylation
K630 Ubiquitination
T639 Phosphorylation
S714 Phosphorylation
K737 Ubiquitination
K744 Ubiquitination
K795 Methylation
K795 Ubiquitination
T798 Phosphorylation
S801 Phosphorylation
K813 Ubiquitination
S819 Phosphorylation
Y823 Phosphorylation
K866 Ubiquitination
Y897 Phosphorylation
S911 Phosphorylation
S913 Phosphorylation
T918 Phosphorylation
Y919 Phosphorylation

研究背景

功能:

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with PCNA.

蛋白家族:

Belongs to the ATP-dependent DNA ligase family.

研究领域

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Base excision repair.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

限制条款

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