(46)
(38)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6701, RRID:AB_2838663.
展开/折叠
Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase;
抗原和靶标
A synthesized peptide derived from human GPX4, corresponding to a region within C-terminal amino acids.
- P36969 GPX4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSLGRLCRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P36969 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S40 | Phosphorylation | Uniprot | |
| K47 | Acetylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot |
研究背景
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (By similarity). Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (By similarity). Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (By similarity). Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (By similarity). The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (By similarity). May be required to protect cells from the toxicity of ingested lipid hydroperoxides (By similarity). Required for normal sperm development and male fertility (By similarity). Essential for maturation and survival of photoreceptor cells (By similarity). Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity).
Mitochondrion.
Cytoplasm.
Present primarily in testis.
Monomer. Has a tendency to form higher mass oligomers.
Belongs to the glutathione peroxidase family.
研究领域
· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)
· Metabolism > Metabolism of other amino acids > Glutathione metabolism.
文献引用
Application: WB Species: Rat Sample:
Application: IF/ICC Species: Rat Sample:
Application: WB Species: Mouse Sample: brain tissues
Application: WB Species: Mouse Sample:
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