(2)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF6765, RRID:AB_2838727.
展开/折叠
1 Cys; 1 Cys peroxiredoxin; 1 Cys PRX; 1 cysPrx; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; 9430088D19Rik; AA690119; Acidic calcium independent phospholipase A2; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; AOP2; Aop2 rs3; Brp 12; Ciliary body glutathione peroxidase; CP 3; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.; Epididymis secretory sperm binding protein Li 128m; GPx; HEL S 128m; KIAA0106; Liver 2D page spot 40; Ltw4; Lvtw 4; MGC46173; mKIAA0106; Non selenium glutathione peroxidase; Non-selenium glutathione peroxidase; NSGPx; ORF06; OTTHUMP00000032693; p29; Peroxiredoxin-6; Peroxiredoxin6; PHGPx; Phospholipase A2 lysosomal; PLA2; PRDX 6; Prdx5; PRDX6; Prdx6 rs3; PRDX6_HUMAN; PRX; Red blood cells page spot 12; Thiol specific antioxidant protein;
抗原和靶标
- P30041 PRDX6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30041 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S32 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| C47 | S-Nitrosylation | Uniprot | |
| K56 | Sumoylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Methylation | Uniprot | |
| K63 | Sumoylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| Y89 | Phosphorylation | Uniprot | |
| C91 | S-Nitrosylation | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| R106 | Methylation | Uniprot | |
| R108 | Methylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Sumoylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K125 | Sumoylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Sumoylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| R155 | Methylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K182 | Acetylation | Uniprot | |
| K182 | Methylation | Uniprot | |
| K182 | Sumoylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K199 | Sumoylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K204 | Sumoylation | Uniprot | |
| K209 | Acetylation | Uniprot | |
| K209 | Sumoylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K216 | Acetylation | Uniprot |
研究背景
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, and can therefore either reduce the oxidized sn-2 fatty acyl grup of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis.
Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress.
Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme.
Cytoplasm. Lysosome.
Note: Also found in lung secretory organelles (lamellar bodies).
Homodimer. Interacts with GSTP1; mediates PRDX6 glutathionylation and regeneration (By similarity). Interacts with APEX1. Interacts with STH. May interact with FAM168B. May interact with HTR2A (By similarity).
Belongs to the peroxiredoxin family. Prx6 subfamily.
研究领域
· Metabolism > Global and overview maps > Metabolic pathways.
文献引用
Application: IHC Species: Human Sample: clinical samples of stomach adenocarcinoma
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