(2)
(4)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0133, RRID:AB_2833317.
展开/折叠
Claudin-10; Claudin10; CLD10_HUMAN; CLDN 10; CLDN10; CLDN10 protein; CPETRL 3; CPETRL3; HGNC:2033; OSP L; OSP like protein; OSP-like protein; OSPL;
抗原和靶标
Expressed in the kidney, eccrine sweat glands and in all layers of the epidermis. In the kidney, it is detected in the thick ascending limb of Henle's loop (TAL) (PubMed:28771254, PubMed:28686597). In the sweat glands, it is expressed in cells from secretory portions, corresponding to the clear cells (PubMed:28686597).
- P78369 CLD10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASTASEIIAFMVSISGWVLVSSTLPTDYWKVSTIDGTVITTATYWANLWKACVTDSTGVSNCKDFPSMLALDGYIQACRGLMIAAVSLGFFGSIFALFGMKCTKVGGSDKAKAKIACLAGIVFILSGLCSMTGCSLYANKITTEFFDPLFVEQKYELGAALFIGWAGASLCIIGGVIFCFSISDNNKTPRYTYNGATSVMSSRTKYHGGEDFKTTNPSKQFDKNAYV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P78369 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S94 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | Uniprot |
研究背景
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in the regulation of paracellular epithelia permeability to ions in multiple organs. It acts as a paracellular ion channel probably forming permselective pores; isoform 1 appears to create pores preferentially permeable to cations and isoform 2 for anions. In sweat glands and in the thick ascending limb (TAL) of Henle's loop in kidney, it controls paracellular sodium permeability which is essential for proper sweat production and renal function.
Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.
Expressed in the kidney, eccrine sweat glands and in all layers of the epidermis. In the kidney, it is detected in the thick ascending limb of Henle's loop (TAL). In the sweat glands, it is expressed in cells from secretory portions, corresponding to the clear cells.
Can form homodimers both in trans (interaction between CLDN10 molecules in opposing membranes) and in cis (interaction between CLDN10 molecules within one membrane).
The fourth transmembrane region (161-181) is necessary for integration into tight junctions.
Belongs to the claudin family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
文献引用
Application: IHC Species: mouse Sample: renal
Application: WB Species: Human Sample: renal tubular epithelial cell
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