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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7043, RRID:AB_2838999.
展开/折叠
CMD1GG; DHSA_HUMAN; Flavoprotein subunit of complex II; Fp; PGL5; SDH 2; SDH1; SDH2; SDHA; SDHF; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Succinate dehydrogenase complex flavoprotein subunit A; Succinate dehydrogenase complex flavoprotein subunit; Succinate dehydrogenase complex flavoprotein subunit precursor; Succinate dehydrogenase complex subunit A; Succinate dehydrogenase complex subunit A flavoprotein (Fp); Succinate dehydrogenase complex subunit A flavoprotein;
抗原和靶标
- P31040 SDHA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGVRGLSRLLSARRLALAKAWPTVLQTGTRGFHFTVDGNKRASAKVSDSISAQYPVVDHEFDAVVVGAGGAGLRAAFGLSEAGFNTACVTKLFPTRSHTVAAQGGINAALGNMEEDNWRWHFYDTVKGSDWLGDQDAIHYMTEQAPAAVVELENYGMPFSRTEDGKIYQRAFGGQSLKFGKGGQAHRCCCVADRTGHSLLHTLYGRSLRYDTSYFVEYFALDLLMENGECRGVIALCIEDGSIHRIRAKNTVVATGGYGRTYFSCTSAHTSTGDGTAMITRAGLPCQDLEFVQFHPTGIYGAGCLITEGCRGEGGILINSQGERFMERYAPVAKDLASRDVVSRSMTLEIREGRGCGPEKDHVYLQLHHLPPEQLATRLPGISETAMIFAGVDVTKEPIPVLPTVHYNMGGIPTNYKGQVLRHVNGQDQIVPGLYACGEAACASVHGANRLGANSLLDLVVFGRACALSIEESCRPGDKVPPIKPNAGEESVMNLDKLRFADGSIRTSELRLSMQKSMQNHAAVFRVGSVLQEGCGKISKLYGDLKHLKTFDRGMVWNTDLVETLELQNLMLCALQTIYGAEARKESRGAHAREDYKVRIDEYDYSKPIQGQQKKPFEEHWRKHTLSYVDVGTGKVTLEYRPVIDKTLNEADCATVPPAIRSY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31040 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T24 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| Y215 | Phosphorylation | P12931 (SRC) | Uniprot |
| S243 | Phosphorylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K335 | Acetylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K361 | Acetylation | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| Y436 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| K498 | Acetylation | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| R500 | Methylation | Uniprot | |
| R507 | Methylation | Uniprot | |
| R512 | Methylation | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| K541 | Acetylation | Uniprot | |
| K541 | Ubiquitination | Uniprot | |
| Y543 | Phosphorylation | P09769 (FGR) | Uniprot |
| K547 | Acetylation | Uniprot | |
| K547 | Methylation | Uniprot | |
| K547 | Ubiquitination | Uniprot | |
| R585 | Methylation | Uniprot | |
| R589 | Methylation | Uniprot | |
| R594 | Methylation | Uniprot | |
| K598 | Acetylation | Uniprot | |
| Y604 | Phosphorylation | P09769 (FGR) | Uniprot |
| Y606 | Phosphorylation | Uniprot | |
| K608 | Acetylation | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| K616 | Ubiquitination | Uniprot | |
| K624 | Acetylation | Uniprot | |
| K624 | Ubiquitination | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| Y629 | Phosphorylation | Uniprot | |
| K647 | Ubiquitination | Uniprot | |
| R662 | Methylation | Uniprot |
研究背景
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.
Acetylated. Deacetylated by SIRT3.
Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.
Component of complex II composed of four subunits: the flavoprotein (FP) SDHA, iron-sulfur protein (IP) SDHB, and a cytochrome b560 composed of SDHC and SDHD (By similarity). Interacts with SDHAF2/SDH5; interaction is required for FAD attachment. Interacts with TRAP1.
Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Energy metabolism > Oxidative phosphorylation.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
文献引用
Application: WB Species: human Sample: ACHN cells
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