TPM2 Antibody - #DF7183

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产品: TPM2 抗体
货号: DF7183
描述: Rabbit polyclonal antibody to TPM2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Sheep, Rabbit, Chicken, Xenopus
分子量: 33kD; 33kD(Calculated).
蛋白号: P07951
RRID: AB_2839135

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IHC 1:50-1:100
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(82%), Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
TPM2 Antibody detects endogenous levels of total TPM2.
RRID:
AB_2839135
引用格式: Affinity Biosciences Cat# DF7183, RRID:AB_2839135.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha tropomyosin; AMCD1; Arthrogryposis multiplex congenital distal type 1; Beta tropomyosin; Beta-tropomyosin; Cytoskeletal tropomyosin TM30; DA1; DA2B; epididymis secretory protein Li 273; FLJ41118; Heat stable cytoskeletal protein 30 kDa; HEL-S-273; hscp30; HTM alpha; hTM5; MGC14582; MGC3261; MGC72094; NEM1; NEM4; Nemaline myopathy type 4; OK/SW cl.5; Sarcomeric tropomyosin kappa; TM 5; TM3; TM30; TM30nm; TMSA; TMSB; TPM 1; TPM 3; TPM1 alpha; TPM1 kappa; TPM2; TPM2_HUMAN; TRK; Tropomyosin 1 alpha; Tropomyosin 1 alpha chain; Tropomyosin 1 alpha chain isoform 6; Tropomyosin 2 (beta); Tropomyosin 2; Tropomyosin 3; Tropomyosin alpha 3 chain; Tropomyosin alpha striated muscle isoform; Tropomyosin beta chain; Tropomyosin gamma; Tropomyosin skeletal muscle beta; Tropomyosin-2;

抗原和靶标

免疫原:
Uniprot:

P07951(TPM2_HUMAN) >>Visit HPA database.

基因/基因ID:
TPM2(7169)
表达:
P07951 TPM2_HUMAN:

Present in primary breast cancer tissue, absent from normal breast tissue.

序列:
MDAIKKKMQMLKLDKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKEAQEKLEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESKCGDLEEELKIVTNNLKSLEAQADKYSTKEDKYEEEIKLLEEKLKEAETRAEFAERSVAKLEKTIDDLEDEVYAQKMKYKAISEELDNALNDITSL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
82
Pig
0
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07951 作为底物

Site PTM Type Enzyme
K12 Acetylation
K12 Ubiquitination
K29 Acetylation
K30 Ubiquitination
K37 Ubiquitination
K48 Acetylation
K48 Ubiquitination
K51 Ubiquitination
T53 Phosphorylation
K59 Ubiquitination
Y60 Phosphorylation
S61 Phosphorylation
S63 Phosphorylation
K65 Ubiquitination
K76 Acetylation
K77 Ubiquitination
T79 Phosphorylation
S87 Phosphorylation
T108 Phosphorylation
K112 Acetylation
K112 Ubiquitination
K118 Acetylation
K118 Ubiquitination
S123 Phosphorylation
K128 Sumoylation
K128 Ubiquitination
K136 Ubiquitination
K140 Acetylation
K149 Ubiquitination
S158 Phosphorylation
Y162 Phosphorylation
K168 Ubiquitination
S188 Phosphorylation
K198 Ubiquitination
K205 Ubiquitination
S206 Phosphorylation
K213 Ubiquitination
K220 Acetylation
K220 Methylation
K220 Ubiquitination
Y221 Phosphorylation
K226 Ubiquitination
K231 Ubiquitination
K233 Ubiquitination
T237 Phosphorylation
S245 Phosphorylation
K248 Acetylation
K248 Ubiquitination
K251 Ubiquitination
T252 Phosphorylation
Y261 Phosphorylation
Y267 Phosphorylation
K268 Ubiquitination
T282 Phosphorylation
S283 Phosphorylation

研究背景

功能:

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.

翻译修饰:

Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization (By similarity).

细胞定位:

Cytoplasm>Cytoskeleton.
Note: Associates with F-actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Present in primary breast cancer tissue, absent from normal breast tissue.

亚基结构:

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain.

蛋白家族:

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Belongs to the tropomyosin family.

研究领域

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

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