SLC5A1 Antibody - #DF7202

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产品: SLC5A1 抗体
货号: DF7202
描述: Rabbit polyclonal antibody to SLC5A1
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 60kDa; 73kD(Calculated).
蛋白号: P13866
RRID: AB_2839154

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Zebrafish(83%), Bovine(100%), Horse(83%), Sheep(100%), Rabbit(83%), Dog(92%), Chicken(92%)
克隆:
Polyclonal
特异性:
SLC5A1 Antibody detects endogenous levels of total SLC5A1.
RRID:
AB_2839154
引用格式: Affinity Biosciences Cat# DF7202, RRID:AB_2839154.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

D22S675; High affinity sodium glucose cotransporter 1; High affinity sodium glucose cotransporter; High affinity sodium-glucose cotransporter; Human Na+/glucose cotransporter 1; Na(+)/glucose cotransporter 1; Na+/glucose cotransporter 1; NAGT; SC5A1_HUMAN; SGLT 1; SGLT1; SLC5A1; Sodium glucose cotransporter 1; Sodium/glucose cotransporter 1; Solute carrier family 5 (sodium/glucose cotransporter) member 1; Solute carrier family 5 member 1;

抗原和靶标

免疫原:
Uniprot:

P13866(SC5A1_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC5A1(6523)
表达:
P13866 SC5A1_HUMAN:

Expressed mainly in intestine and kidney.

描述:
This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene.
序列:
MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Pig
92
Dog
92
Chicken
92
Horse
83
Zebrafish
83
Rabbit
83
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13866 作为底物

Site PTM Type Enzyme
Y236 Phosphorylation
T242 Phosphorylation
N248 N-Glycosylation
T271 Phosphorylation
S335 Phosphorylation
S418 Phosphorylation
S437 Phosphorylation
T587 Phosphorylation
Y606 Phosphorylation

研究背景

功能:

Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.

翻译修饰:

N-glycosylation is not necessary for the cotransporter function.

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed mainly in intestine and kidney.

蛋白家族:

Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

研究领域

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). Phlorizin Exerts Direct Protective Effects on Palmitic Acid (PA)-Induced Endothelial Dysfunction by Activating the PI3K/AKT/eNOS Signaling Pathway and Increasing the Levels of Nitric Oxide (NO). Medical Science Monitor Basic Research (PubMed: 29307883) [IF=2.8]

Application: WB    Species: human    Sample: HUVECs

Figure 1.| PA stimulates expression of SGLT1 and SGLT2 in HUVECs. Cells were incubated for 18 h in the presence of PA (300 µM). (A, B) SGLT1 and SGLT2 proteins in the samples were analyzed by Western blotting. Bar plots show the summarized data of the relative density after being normalized to GAPDH and are expressed as average percentage of control ±SEM. (C, D) Quantitative PCR analysis of mRNA expression of SGLT1 and SGLT2, normalized to GAPDH mRNA. ** P<0.01.

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