(2)
(5)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7325, RRID:AB_2839263.
展开/折叠
6-bisphosphatase 1; 6-bisphosphate 1-phosphohydrolase 1; D fructose 1 6 bisphosphate 1 phosphohydrolase 1; D-fructose-1; EC 3.1.3.11; F16P1_HUMAN; FBP; FBP 1; FBP1; FBPase 1; Fructose 1 6 bisphosphatase 1; Fructose bisphosphatase 1; Fructose-1; Growth inhibiting protein 17; Liver fructose bisphosphatase;
抗原和靶标
- P09467 F16P1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P09467 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T28 | Phosphorylation | Uniprot | |
| Y58 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K72 | Acetylation | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| Y216 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | Uniprot | |
| K232 | Acetylation | Uniprot | |
| Y241 | Phosphorylation | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| Y259 | Phosphorylation | Uniprot | |
| Y265 | Phosphorylation | Uniprot | |
| K269 | Acetylation | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| T297 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| K330 | Acetylation | Uniprot |
研究背景
Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.
Expressed in pancreatic islets.
Homotetramer.
Belongs to the FBPase class 1 family.
研究领域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.
· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
文献引用
Application: WB Species: Human Sample: renal tissues
限制条款
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