| 产品: | FH 抗体 |
| 货号: | DF7460 |
| 描述: | Rabbit polyclonal antibody to FH |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Rabbit, Dog |
| 分子量: | 54kDa; 55kD(Calculated). |
| 蛋白号: | P07954 |
| RRID: | AB_2839397 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7460, RRID:AB_2839397.
展开/折叠
FH; Fumarase; Fumarate hydratase; Fumarate hydratase mitochondrial; Fumarate hydratase, mitochondrial; FUMH_HUMAN; HLRCC; LRCC; MCL; MCUL 1; MCUL1; MS709; Multiple hereditary cutaneous leiomyomata;
抗原和靶标
Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.
- P07954 FUMH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYRALRLLARSRPLVRAPAAALASAPGLGGAAVPSFWPPNAARMASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P07954 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S46 | Phosphorylation | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| K61 | Acetylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| Y68 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| K80 | Acetylation | Uniprot | |
| T90 | Phosphorylation | Uniprot | |
| K94 | Acetylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K100 | Methylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| K115 | Acetylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K122 | Acetylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| K172 | Acetylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| T236 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| Y251 | Phosphorylation | Uniprot | |
| K256 | Acetylation | Uniprot | |
| K292 | Acetylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| R343 | Methylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| Y465 | Phosphorylation | Uniprot | |
| K467 | Acetylation | Uniprot | |
| K470 | Acetylation | Uniprot | |
| K473 | Acetylation | Uniprot | |
| K477 | Acetylation | Uniprot | |
| Y491 | Phosphorylation | Uniprot | |
| T493 | Phosphorylation | Uniprot | |
| K502 | Ubiquitination | Uniprot |
研究背景
Catalyzes the reversible stereospecific interconversion of fumarate to L-malate. Experiments in other species have demonstrated that specific isoforms of this protein act in defined pathways and favor one direction over the other (Probable).
Catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle to facilitate a transition step in the production of energy in the form of NADH.
Catalyzes the dehydration of L-malate to fumarate (By similarity). Fumarate metabolism in the cytosol plays a role during urea cycle and arginine metabolism; fumarate being a by-product of the urea cycle and amino-acid catabolism (By similarity). Also plays a role in DNA repair by promoting non-homologous end-joining (NHEJ). In response to DNA damage and phosphorylation by PRKDC, translocates to the nucleus and accumulates at DNA double-strand breaks (DSBs): acts by catalyzing formation of fumarate, an inhibitor of KDM2B histone demethylase activity, resulting in enhanced dimethylation of histone H3 'Lys-36' (H3K36me2).
Phosphorylation at Thr-236 by PRKDC in response to DNA damage promotes translocation to the nucleus and recruitment to DNA double-strand breaks (DSBs).
Mitochondrion.
Cytoplasm>Cytosol. Nucleus. Chromosome.
Note: Translocates to the nucleus in response to DNA damage: localizes to DNA double-strand breaks (DSBs) following phosphorylation by PRKDC.
Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.
Homotetramer. Interacts with H2AZ1.
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
研究领域
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
限制条款
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