| 产品: | EHMT1 抗体 |
| 货号: | DF2491 |
| 描述: | Rabbit polyclonal antibody to EHMT1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 170 kDa; 141kD(Calculated). |
| 蛋白号: | Q9H9B1 |
| RRID: | AB_2839697 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF2491, RRID:AB_2839697.
展开/折叠
bA188C12.1; DKFZp667M072; EHMT 1; EHMT1; EHMT1_HUMAN; Eu-HMTase1; Euchromatic histone lysine N methyltransferase 1; Euchromatic histone methyltransferase 1; Euchromatic histone-lysine N-methyltransferase 1; EUHMTASE1; FLJ12879; FP13812; G9a like protein 1; G9a like protein; G9a-like protein 1; GLP 1; GLP; GLP1; H3 K9 HMTase 5; H3-K9-HMTase 5; Histone H3 K9 methyltransferase 5; Histone H3-K9 methyltransferase 5; Histone lysine N methyltransferase H3 lysine 9 specific 5; Histone-lysine N-methyltransferase EHMT1; KIAA1876; Lysine N methyltransferase 1D; Lysine N-methyltransferase 1D; RP11 188C12.1;
抗原和靶标
- Q9H9B1 EHMT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAADAEAVPARGEPQQDCCVKTELLGEETPMAADEGSAEKQAGEAHMAADGETNGSCENSDASSHANAAKHTQDSARVNPQDGTNTLTRIAENGVSERDSEAAKQNHVTADDFVQTSVIGSNGYILNKPALQAQPLRTTSTLASSLPGHAAKTLPGGAGKGRTPSAFPQTPAAPPATLGEGSADTEDRKLPAPGADVKVHRARKTMPKSVVGLHAASKDPREVREARDHKEPKEEINKNISDFGRQQLLPPFPSLHQSLPQNQCYMATTKSQTACLPFVLAAAVSRKKKRRMGTYSLVPKKKTKVLKQRTVIEMFKSITHSTVGSKGEKDLGASSLHVNGESLEMDSDEDDSEELEEDDGHGAEQAAAFPTEDSRTSKESMSEADRAQKMDGESEEEQESVDTGEEEEGGDESDLSSESSIKKKFLKRKGKTDSPWIKPARKRRRRSRKKPSGALGSESYKSSAGSAEQTAPGDSTGYMEVSLDSLDLRVKGILSSQAEGLANGPDVLETDGLQEVPLCSCRMETPKSREITTLANNQCMATESVDHELGRCTNSVVKYELMRPSNKAPLLVLCEDHRGRMVKHQCCPGCGYFCTAGNFMECQPESSISHRFHKDCASRVNNASYCPHCGEESSKAKEVTIAKADTTSTVTPVPGQEKGSALEGRADTTTGSAAGPPLSEDDKLQGAASHVPEGFDPTGPAGLGRPTPGLSQGPGKETLESALIALDSEKPKKLRFHPKQLYFSARQGELQKVLLMLVDGIDPNFKMEHQNKRSPLHAAAEAGHVDICHMLVQAGANIDTCSEDQRTPLMEAAENNHLEAVKYLIKAGALVDPKDAEGSTCLHLAAKKGHYEVVQYLLSNGQMDVNCQDDGGWTPMIWATEYKHVDLVKLLLSKGSDINIRDNEENICLHWAAFSGCVDIAEILLAAKCDLHAVNIHGDSPLHIAARENRYDCVVLFLSRDSDVTLKNKEGETPLQCASLNSQVWSALQMSKALQDSAPDRPSPVERIVSRDIARGYERIPIPCVNAVDSEPCPSNYKYVSQNCVTSPMNIDRNITHLQYCVCIDDCSSSNCMCGQLSMRCWYDKDGRLLPEFNMAEPPLIFECNHACSCWRNCRNRVVQNGLRARLQLYRTRDMGWGVRSLQDIPPGTFVCEYVGELISDSEADVREEDSYLFDLDNKDGEVYCIDARFYGNVSRFINHHCEPNLVPVRVFMAHQDLRFPRIAFFSTRLIEAGEQLGFDYGERFWDIKGKLFSCRCGSPKCRHSSAALAQRQASAAQEAQEDGLPDTSSAAAADPL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H9B1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K22 | Sumoylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| T110 | Phosphorylation | Uniprot | |
| K129 | Acetylation | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| K153 | Methylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| T171 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K219 | Acetylation | Uniprot | |
| K234 | Sumoylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K301 | Acetylation | Uniprot | |
| K302 | Acetylation | Uniprot | |
| K303 | Acetylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| S348 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| K432 | Methylation | Uniprot | |
| K432 | Sumoylation | Uniprot | |
| T433 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| K439 | Ubiquitination | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| K451 | Sumoylation | Uniprot | |
| Y479 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| S497 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| Y560 | Phosphorylation | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| K644 | Sumoylation | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| T652 | Phosphorylation | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| T708 | Phosphorylation | Uniprot | |
| S729 | Phosphorylation | Uniprot | |
| K731 | Ubiquitination | Uniprot | |
| K740 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| K890 | Ubiquitination | Uniprot | |
| K895 | Ubiquitination | Uniprot | |
| Y952 | Phosphorylation | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| R1002 | Methylation | Uniprot | |
| S1004 | Phosphorylation | Uniprot | |
| S1048 | Phosphorylation | Uniprot | |
| K1086 | Ubiquitination | Uniprot | |
| S1276 | Phosphorylation | Uniprot |
研究背景
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.
Nucleus. Chromosome.
Note: Associates with euchromatic regions.
Widely expressed.
Heterodimer; heterodimerizes with EHMT2. Interacts with WIZ and EHMT2. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts (via ANK repeats) with RELA (when monomethylated at 'Lys-310'). Interacts with MPHOSPH8. Interacts with CDYL. Interacts with REST only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2.
The ANK repeats recognize and bind RELA subunit of NF-kappa-B, when RELA is monomethylated at 'Lys-310' (By similarity). They also specifically recognize and bind H3K9me1 and H3K9me2.
The SET domain mediates interaction with WIZ.
In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
Belongs to the class V-like SAM-binding methyltransferase superfamily.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
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