CCR1 Antibody - #DF2710

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产品: CCR1 抗体
货号: DF2710
描述: Rabbit polyclonal antibody to CCR1
应用: WB IHC
反应: Human
预测: Bovine, Horse, Sheep
分子量: 37.6 kDa; 41kD(Calculated).
蛋白号: P32246
RRID: AB_2839916

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Bovine(80%), Horse(90%), Sheep(80%)
克隆:
Polyclonal
特异性:
CCR1 Antibody detects endogenous levels of total CCR1.
RRID:
AB_2839916
引用格式: Affinity Biosciences Cat# DF2710, RRID:AB_2839916.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C C chemokine receptor type 1; C C CKR 1; C-C chemokine receptor type 1; C-C CKR-1; CC CKR 1; CC-CKR-1; CCR 1; CCR-1; CCR1; CCR1_HUMAN; CD 191; CD191; CD191 antigen; Chemokine (C C motif) receptor 1; Chemokine C C motif receptor 1; CKR 1; CKR1; CMKBR 1; CMKBR1; CMKR 1; CMKR1; HM145; LD78 receptor; Macrophage inflammatory protein 1 alpha /Rantes receptor; Macrophage inflammatory protein 1 alpha receptor; Macrophage inflammatory protein 1-alpha receptor; Mip 1a R; MIP 1alpha R; MIP-1alpha-R; MIP1aR; RANTES R; RANTES receptor; RANTES-R; SCYAR1;

抗原和靶标

免疫原:
Uniprot:

P32246(CCR1_HUMAN) >>Visit HPA database.

基因/基因ID:
CCR1(1230)
表达:
P32246 CCR1_HUMAN:

Widely expressed in different hematopoietic cells.

描述:
Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.;
序列:
METPNTTEDYDTTTEFDYGDATPCQKVNERAFGAQLLPPLYSLVFVIGLVGNILVVLVLVQYKRLKNMTSIYLLNLAISDLLFLFTLPFWIDYKLKDDWVFGDAMCKILSGFYYTGLYSEIFFIILLTIDRYLAIVHAVFALRARTVTFGVITSIIIWALAILASMPGLYFSKTQWEFTHHTCSLHFPHESLREWKLFQALKLNLFGLVLPLLVMIICYTGIIKILLRRPNEKKSKAVRLIFVIMIIFFLFWTPYNLTILISVFQDFLFTHECEQSRHLDLAVQVTEVIAYTHCCVNPVIYAFVGERFRKYLRQLFHRRVAVHLVKWLPFLSVDRLERVSSTSPSTGEHELSAGF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
90
Bovine
80
Sheep
80
Pig
60
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P32246 作为底物

Site PTM Type Enzyme
Y41 Phosphorylation
K326 Ubiquitination
S332 Phosphorylation
S340 Phosphorylation
S341 Phosphorylation
T342 Phosphorylation
S343 Phosphorylation
S345 Phosphorylation
T346 Phosphorylation
S352 Phosphorylation

研究背景

功能:

Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed in different hematopoietic cells.

亚基结构:

Interacts with CREB3.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

文献引用

1). Bioinformatics Analysis for Identification of Key Genes in Salivary Gland and the Potential of a Combination of Biomarkers for the Diagnosis of SS. Journal of Inflammation Research (PubMed: 34475773) [IF=4.5]

Application: IF/ICC    Species: Human    Sample: gland tissue

Figure 5 H&E and immunohistochemical analysis (A) of STAT1, MNDA, IL10RA, and CCR1 proteins in minor salivary gland biopsy (MSGB) staining from 14 SS patients and 14 non-SS patients (magnification: ×200 for H&E staining and ×200 for IHC). Blue marks the nucleus, and brown marks the target protein. The bar graph indicated medians with interquartile ranges of IOD value (B). ***P<0.001.

Application: IHC    Species: human    Sample: Labial Gland Tissues

Figure 5| H&E and immunohistochemical analysis (A) of STAT1, MNDA, IL10RA, and CCR1 proteins in minor salivary gland biopsy (MSGB) staining from 14 SS patients and 14 non-SS patients (magnification: ×200 for H&E staining and ×200 for IHC). Blue marks the nucleus, and brown marks the target protein. The bar graph indicated medians with interquartile ranges of IOD value (B). ***P<0.001.
2). High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells. American Journal of Translational Research (PubMed: 30788000) [IF=2.2]

Application: IHC    Species: human    Sample: primary NSCLC and bone metastasis tissues

Figure 2. |Expression of possible target chemokine receptors of CCL7 in primary NSCLC and bone metastasis tissues. A-C. IHC detection of CCR1, CCR2 and CCR3 expression in primary NSCLC and bone metastasis tissues. CCR1, CCR2 and CCR3 were mainly expressed on the cell membrane and in cytoplasm (arrow). D. qRT-PCR measurement of mRNA levels of CCR1, CCR2, CCR3 in primary NSCLC and bone metastasis tissues. *: P < 0.05.

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