PIAS1 Antibody - #AF0561

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产品: PIAS1 抗体
货号: AF0561
描述: Rabbit polyclonal antibody to PIAS1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 72kDa; 72kD(Calculated).
蛋白号: O75925
RRID: AB_2834162

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
PIAS1 Antibody detects endogenous levels of total PIAS1.
RRID:
AB_2834162
引用格式: Affinity Biosciences Cat# AF0561, RRID:AB_2834162.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AR interacting protein; DDXBP1; DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1; DEAD/H box binding protein 1; DEAD/H box-binding protein 1; E3 SUMO-protein ligase PIAS1; GBP; Gu binding protein; Gu-binding protein; GU/RH-II; Pias1; PIAS1_HUMAN; Protein inhibitor of activated STAT protein 1; Protein inhibitor of activated STAT, 1; RNA helicase II binding protein; RNA helicase II-binding protein; Zinc finger, MIZ-type containing 3; ZMIZ3;

抗原和靶标

免疫原:
Uniprot:

O75925(PIAS1_HUMAN) >>Visit HPA database.

基因/基因ID:
PIAS1(8554)
表达:
O75925 PIAS1_HUMAN:

Expressed in numerous tissues with highest level in testis.

描述:
PIAS1 Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified).
序列:
MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGTWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISLD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75925 作为底物

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
K8 Sumoylation
K8 Ubiquitination
S13 Phosphorylation
S17 Phosphorylation P49841 (GSK3B)
K30 Ubiquitination
K34 Ubiquitination
K40 Sumoylation
K40 Ubiquitination
K46 Sumoylation
K46 Ubiquitination
S50 Phosphorylation
K56 Sumoylation
K56 Ubiquitination
K58 Sumoylation
K58 Ubiquitination
T71 Phosphorylation
S90 Phosphorylation
K117 Sumoylation
K137 Sumoylation
K137 Ubiquitination
K140 Ubiquitination
K152 Sumoylation
K152 Ubiquitination
K226 Ubiquitination
K238 Sumoylation
K238 Ubiquitination
S254 Phosphorylation
S289 Phosphorylation
S290 Phosphorylation
T291 Phosphorylation
R303 Methylation
S308 Phosphorylation
K313 Ubiquitination
K315 Sumoylation
K315 Ubiquitination
T327 Phosphorylation
S328 Phosphorylation
K339 Ubiquitination
K369 Ubiquitination
K408 Ubiquitination
K419 Ubiquitination
K420 Ubiquitination
K453 Ubiquitination
T463 Phosphorylation
S466 Phosphorylation P68400 (CSNK2A1)
S467 Phosphorylation P68400 (CSNK2A1)
S468 Phosphorylation P68400 (CSNK2A1)
S476 Phosphorylation
T480 Phosphorylation
S483 Phosphorylation
S485 Phosphorylation
T487 Phosphorylation
S488 Phosphorylation
K493 Ubiquitination
S497 Phosphorylation
S503 Phosphorylation
S506 Phosphorylation
T508 Phosphorylation
S510 Phosphorylation
S522 Phosphorylation P49137 (MAPKAPK2)

研究背景

功能:

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity).

翻译修饰:

Sumoylated.

细胞定位:

Nucleus speckle. Nucleus>PML body.
Note: Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in numerous tissues with highest level in testis.

亚基结构:

Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation (By similarity). Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Interacts with PML (By similarity). Interacts with MTA1. Interacts with SUMO1P1/SUMO5.

蛋白家族:

The LXXLL motif is a transcriptional coregulator signature.

The SP-RING-type domain is required for promoting EKLF sumoylation.

Belongs to the PIAS family.

研究领域

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

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