产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0106, RRID:AB_2833633.
展开/折叠
AMF; Aurocrine motility factor; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; G6PI_HUMAN; Glucose phosphate isomerase; Glucose-6-phosphate isomerase; GNPI; GPI; Gpi1; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; NLK; Oxoisomerase; PGI; PHI; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; Phosphosaccharomutase; SA 36; SA-36; SA36; Sperm antigen 36;
抗原和靶标
Purified recombinant fragment of human Glucose-6-phosphate isomerase expressed in E. Coli.
- P06744 G6PI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ
翻译修饰 - P06744 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K12 | Methylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| R66 | Methylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| T93 | Phosphorylation | Uniprot | |
| R106 | Methylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| K116 | Acetylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K124 | Acetylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K142 | Acetylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| Y174 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| Y183 | Phosphorylation | Uniprot | |
| S185 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K194 | Ubiquitination | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| K211 | Acetylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| T214 | Phosphorylation | Uniprot | |
| T215 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K234 | Acetylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| T248 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| K252 | Acetylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| K362 | Acetylation | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| C404 | S-Nitrosylation | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| R438 | Methylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K454 | Acetylation | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| S455 | Phosphorylation | Uniprot | |
| K466 | Acetylation | Uniprot | |
| K466 | Sumoylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| S532 | Phosphorylation | Uniprot |
研究背景
In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
ISGylated.
Cytoplasm. Secreted.
Homodimer; in the catalytically active form. Monomer in the secreted form.
Belongs to the GPI family.
研究领域
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.
· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
限制条款
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