| 产品: | DNAJC3 抗体 |
| 货号: | DF7685 |
| 描述: | Rabbit polyclonal antibody to DNAJC3 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 54 kDa; 58kD(Calculated). |
| 蛋白号: | Q13217 |
| RRID: | AB_2841156 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7685, RRID:AB_2841156.
展开/折叠
DnaJ (Hsp40) homolog subfamily C member 3; DnaJ homolog subfamily C member 3; DNAJC3; DNJC3_HUMAN; double-stranded RNA-activated protein kinase inhibitor; Endoplasmic reticulum DnaJ protein 6; ERdj6; HP58; Interferon induced, double stranded RNA activated protein kinase inhibitor; Interferon-induced; P58; p58 ipk; P58IPK; PRKRI; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58; Protein kinase interferon inducible double stranded RNA dependent inhibitor;
抗原和靶标
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
- Q13217 DNJC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVAPGSVTSRLGSVFPFLLVLVDLQYEGAECGVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYDEAIQDYETAQEHNENDQQIREGLEKAQRLLKQSQKRDYYKILGVKRNAKKQEIIKAYRKLALQWHPDNFQNEEEKKKAEKKFIDIAAAKEVLSDPEMRKKFDDGEDPLDAESQQGGGGNPFHRSWNSWQGFNPFSSGGPFRFKFHFN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13217 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K46 | Methylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| K407 | Methylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| K457 | Ubiquitination | Uniprot |
研究背景
Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Endoplasmic reticulum.
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
Interacts with EIF2AK4/GCN2; this interaction occurs under endoplasmic reticulum (ER) stress, hypothermic and amino acid starving stress conditions and inhibits EIF2AK4/GCN2 kinase activity. Interacts with EIF2AK3 (By similarity). Interacts with EIF2AK2. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with THAP12.
The J domain mediates interaction with HSPA8.
Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Viral > Influenza A.
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