产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF7947, RRID:AB_2841349.
展开/折叠
A330085O09Rik; Caspase activated deoxyribonuclease inhibitor short form; DFF 1; DFF 45; DFF alpha; DFF-45; DFF1; DFF35; DFF45; DFFA; Dffa DNA fragmentation factor, alpha subunit; DFFA_HUMAN; DNA fragmentation factor 45 kDa subunit; DNA Fragmentation Factor Alpha Subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45 kD, alpha subunit; DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, alpha subunit; DNAation factor 45 kDa subunit; H13; ICAD; ICAD L; ICAD S; Inhibitor of CAD; Inhibitor of Caspase Activated DNase; MGC143066; OTTHUMP00000001903; OTTHUMP00000001904; RP23 121D17.3;
抗原和靶标
- O00273 DFFA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O00273 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| Y27 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| Y75 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| R296 | Methylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K324 | Acetylation | Uniprot |
研究背景
Inhibitor of the caspase-activated DNase (DFF40).
Caspase-3 cleaves DFF45 at 2 sites to generate an active factor.
Cytoplasm.
Heterodimer of DFFA and DFFB.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
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