产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8249, RRID:AB_2841543.
展开/折叠
Amplified in osteosarcoma 9; amplified in osteosarcoma; ERLEC2; OS-9; Os9; OS9_HUMAN; Osteosarcoma amplified 9 endoplasmic reticulum lectin; Protein OS-9;
抗原和靶标
Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
- Q13438 OS9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIEILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELLSPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAKASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSYVLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEELQDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQDPSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDAAEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRDRLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQSPELVKKHKKKRVVPKKPPPSPQPTEEDPEHRVRVRVTKLRLGGPNQDLTVLEMKRENPQLKQIEGLVKELLEREGLTAAGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEIFFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13438 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K113 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| K514 | Ubiquitination | Uniprot | |
| S529 | O-Glycosylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| T533 | O-Glycosylation | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| K619 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K641 | Ubiquitination | Uniprot |
研究背景
Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
Intramolecular disulfide bonds.
Isoform 1 and isoform 2 are N-glycosylated.
Endoplasmic reticulum lumen.
Ubiquitously expressed. Found as well in all tumor cell lines analyzed, amplified in sarcomas. Highly expressed in osteosarcoma SJSA-1 and rhabdomyosarcoma Rh30 cell lines. Isoform 2 is the major isoform detected in all cell types examined.
Interacts (via C-terminus) with CPNE6 (via second C2 domain); this interaction occurs in a calcium-dependent manner in vitro (By similarity). Probably part of the HRD1 ubiquitin ligase complex composed at least of SYVN1/HRD1 and SEL1L with which it interacts directly. Through this complex it may interact with ERLEC1 and HSPA5. Interacts with DERL2. Interacts with HSP90B1. Interacts with CREB3. Interacts with SYVN1.
Belongs to the OS-9 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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