| 产品: | LMAN1 抗体 |
| 货号: | DF8460 |
| 描述: | Rabbit polyclonal antibody to LMAN1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 58 kDa; 58kD(Calculated). |
| 蛋白号: | P49257 |
| RRID: | AB_2841696 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8460, RRID:AB_2841696.
展开/折叠
Endoplasmic reticulum golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; ERGIC-53; ERGIC53; ERGIC53 like protein; F5F8D; FMFD1; Gp58; Intracellular mannose specific lectin; Intracellular mannose-specific lectin MR60; Lectin mannose binding 1; Lectin mannose-binding 1; Lman1; LMAN1 like protein; LMAN1_HUMAN; MCFD1; MR60; Protein ERGIC-53;
抗原和靶标
- P49257 LMAN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGSRQRGLRARVRPLFCALLLSLGRFVRGDGVGGDPAVALPHRRFEYKYSFKGPHLVQSDGTVPFWAHAGNAIPSSDQIRVAPSLKSQRGSVWTKTKAAFENWEVEVTFRVTGRGRIGADGLAIWYAENQGLEGPVFGSADLWNGVGIFFDSFDNDGKKNNPAIVIIGNNGQIHYDHQNDGASQALASCQRDFRNKPYPVRAKITYYQNTLTVMINNGFTPDKNDYEFCAKVENMIIPAQGHFGISAATGGLADDHDVLSFLTFQLTEPGKEPPTPDKEISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPAEEIFESVGDRELRQVFEGQNRIHLEIKQLNRQLDMILDEQRRYVSSLTEEISKRGAGMPGQHGQITQQELDTVVKTQHEILRQVNEMKNSMSETVRLVSGMQHPGSAGGVYETTQHFIDIKEHLHIVKRDIDNLVQRNMPSNEKPKCPELPPFPSCLSTVHFIIFVVVQTVLFIGYIMYRSQQEAAAKKFF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P49257 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K49 | Ubiquitination | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| T276 | O-Glycosylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| T385 | O-Glycosylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S425 | O-Glycosylation | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| Y430 | Phosphorylation | Uniprot | |
| T432 | O-Glycosylation | Uniprot | |
| T433 | O-Glycosylation | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot |
研究背景
Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins.
The N-terminal may be partly blocked.
Endoplasmic reticulum-Golgi intermediate compartment membrane>Single-pass type I membrane protein. Golgi apparatus membrane>Single-pass membrane protein. Endoplasmic reticulum membrane>Single-pass type I membrane protein.
Ubiquitous.
Exists both as a covalent disulfide-linked homohexamer, and a complex of three disulfide-linked dimers non-covalently kept together. Interacts with MCFD2. May interact with TMEM115. Interacts with RAB3GAP1 and RAB3GAP2. Interacts with UBXN6.
The FF ER export motif at the C-terminus is not sufficient to support endoplasmic reticulum exit, and needs assistance of Gln-501 for proper recognition of COPII coat components.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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