| 产品: | TAF10 抗体 |
| 货号: | DF8819 |
| 描述: | Rabbit polyclonal antibody to TAF10 |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Rabbit, Dog |
| 分子量: | 22 kDa; 22kD(Calculated). |
| 蛋白号: | Q12962 |
| RRID: | AB_2842016 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8819, RRID:AB_2842016.
展开/折叠
dTAF(II)24; TAF24; TAF2H; TAFII24; TAFII30; Transcription initiation factor TFIID 24 kDa subunit; Transcription initiation factor TFIID subunit 10;
抗原和靶标
- Q12962 TAF10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSCSGSGADPEAAPASAASAPGPAPPVSAPAALPSSTAAENKASPAGTAGGPGAGAAAGGTGPLAARAGEPAERRGAAPVSAGGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFLMQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTASGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12962 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| T37 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| R67 | Methylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| K187 | Acetylation | Uniprot | |
| K189 | Methylation | Uniprot | |
| Y207 | Phosphorylation | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| Y216 | Phosphorylation | Uniprot |
研究背景
TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors.
Monomethylated at Lys-189 by SETD7, leading to increased affinity for RNA polymerase II.
Lysine deamination at Lys-189 to form allysine is mediated by LOXL2. Allysine formation by LOXL2 results in release of TAF10 from promoters, leading to inhibition of TFIID-dependent transcription.
Nucleus.
TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, SUPT3H, TAF5L TAF6L, TAF9, TAF10, TAF12 and TRRAP. Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Interacts with TAF3. Interacts with LOXL2.
The [KR]-[STA]-K motif is specifically recognized by the SETD7 methyltransferase.
Belongs to the TAF10 family.
研究领域
· Genetic Information Processing > Transcription > Basal transcription factors.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
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