产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8940, RRID:AB_2842136.
展开/折叠
DJ 2; DJ2; DjA1; DnaJ (Hsp40) homolog, subfamily A, member 1; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; DNAJ2; Dnaja1; DNJA1_HUMAN; hDJ 2; HDJ-2; HDJ2; Heat shock 40 kDa protein 4; heat shock protein DNAJ like 2; Heat shock protein J2; HSDJ; HSJ-2; HSJ2; HSPF4; Human DnaJ protein 2; NEDD7; Neural precursor cell expressed developmentally down regulated 7; OTTHUMP00000021193;
抗原和靶标
Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
- P31689 DNJA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P31689 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Ubiquitination | Uniprot | |
| Y7 | Phosphorylation | Uniprot | |
| Y8 | Phosphorylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K44 | Acetylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K136 | Acetylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| R253 | Methylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| C302 | S-Nitrosylation | Uniprot | |
| Y311 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| Y376 | Phosphorylation | Uniprot | |
| Y381 | Phosphorylation | Uniprot |
研究背景
Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV.
Membrane>Lipid-anchor. Cytoplasm. Microsome. Nucleus. Cytoplasm>Perinuclear region. Mitochondrion.
Note: Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.
Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver.
Identified in a complex with HSPA1B and BAX. Interacts with RNF207.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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