| 产品: | BRAP 抗体 |
| 货号: | DF9289 |
| 描述: | Rabbit polyclonal antibody to BRAP |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 67 kDa; 67kD(Calculated). |
| 蛋白号: | Q7Z569 |
| RRID: | AB_2842485 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9289, RRID:AB_2842485.
展开/折叠
3010002G07Rik; BRAP2; BRCA1 associated protein; EC 6.3.2.; Galectin 2 binding protein; IMP; Impedes mitogenic signal propagation; Renal carcinoma antigen NY REN 63; RING finger protein 52; RNF52; zgc:92894;
抗原和靶标
- Q7Z569 BRAP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVSLVVIRLELAEHSPVPAGFGFSAAAGEMSDEEIKKTTLASAVACLEGKSPGEKVAIIHQHLGRREMTDVIIETMKSNPDELKTTVEERKSSEASPTAQRSKDHSKECINAAPDSPSKQLPDQISFFSGNPSVEIVHGIMHLYKTNKMTSLKEDVRRSAMLCILTVPAAMTSHDLMKFVAPFNEVIEQMKIIRDSTPNQYMVLIKFRAQADADSFYMTCNGRQFNSIEDDVCQLVYVERAEVLKSEDGASLPVMDLTELPKCTVCLERMDESVNGILTTLCNHSFHSQCLQRWDDTTCPVCRYCQTPEPVEENKCFECGVQENLWICLICGHIGCGRYVSRHAYKHFEETQHTYAMQLTNHRVWDYAGDNYVHRLVASKTDGKIVQYECEGDTCQEEKIDALQLEYSYLLTSQLESQRIYWENKIVRIEKDTAEEINNMKTKFKETIEKCDNLEHKLNDLLKEKQSVERKCTQLNTKVAKLTNELKEEQEMNKCLRANQVLLQNKLKEEERVLKETCDQKDLQITEIQEQLRDVMFYLETQQKINHLPAETRQEIQEGQINIAMASASSPASSGGSGKLPSRKGRSKRGK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q7Z569 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K38 | Ubiquitination | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| T173 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| T352 | Phosphorylation | Uniprot | |
| T361 | Phosphorylation | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| Y422 | Phosphorylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| K458 | Acetylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K464 | Ubiquitination | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| K507 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| K580 | Ubiquitination | Uniprot |
研究背景
Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.
Cytoplasm.
Expressed in breast epithelial cell lines.
Interacts with the nuclear localization signal of BRCA1 and with the N-terminal of KSR1. The C-terminal portion of BCRA1 interacts with DDB1.
研究领域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
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