产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9490, RRID:AB_2842686.
展开/折叠
E3 ubiquitin protein ligase RNF5; E3 ubiquitin-protein ligase RNF5; G16; HsRma1; NG2; OTTHUMP00000029107; Protein G16; Ram1 homolog; RING finger protein 5; RING5; RMA1; RNF 5; RNF5; RNF5_HUMAN;
抗原和靶标
- Q99942 RNF5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGTGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99942 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot |
研究背景
Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of STING1 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurs in mitochondria after viral transfection and regulates antiviral responses.
Membrane>Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane.
Note: Predominantly located in the plasma membrane, with some localization occurring within cytoplasmic organelles.
Widely expressed.
Interacts with PXN. Interacts with Salmonella typhimurium sopA. Interacts with JKAMP. Interacts with STING1; the interaction of endogenous proteins is dependent on viral infection.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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