产品: SHP1 抗体
货号: AF7787
描述: Rabbit polyclonal antibody to SHP1
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 67kDa; 68kD(Calculated).
蛋白号: P29350
RRID: AB_2844151

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Zebrafish(83%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(100%), Dog(90%)
克隆:
Polyclonal
特异性:
SHP1 Antibody detects endogenous levels of total SHP1.
RRID:
AB_2844151
引用格式: Affinity Biosciences Cat# AF7787, RRID:AB_2844151.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP 1; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; Ptpn6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1; SHP1L; tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P29350 PTN6_HUMAN:

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

序列:
MVRWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
90
Horse
90
Bovine
90
Sheep
90
Dog
90
Zebrafish
83
Chicken
78
Xenopus
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P29350 作为底物

Site PTM Type Enzyme
S10 Phosphorylation
K19 Ubiquitination
S26 Phosphorylation
K34 Ubiquitination
S57 Phosphorylation
Y61 Phosphorylation
Y64 Phosphorylation
K97 Ubiquitination
Y98 Phosphorylation
Y111 Phosphorylation
S140 Phosphorylation
K155 Ubiquitination
K168 Ubiquitination
Y176 Phosphorylation
T177 Phosphorylation
S186 Phosphorylation
T188 Phosphorylation
K195 Ubiquitination
K196 Ubiquitination
Y208 Phosphorylation
Y213 Phosphorylation
Y214 Phosphorylation
K232 Ubiquitination
K241 Ubiquitination
S250 Phosphorylation
K253 Ubiquitination
K257 Ubiquitination
K271 Ubiquitination
Y276 Phosphorylation
K277 Acetylation
K277 Ubiquitination
S285 Phosphorylation
R286 Methylation
S299 Phosphorylation
Y301 Phosphorylation
Y306 Phosphorylation
K308 Ubiquitination
K360 Ubiquitination
Y364 Phosphorylation
Y374 Phosphorylation
Y377 Phosphorylation
S378 Phosphorylation
K391 Ubiquitination
T394 Phosphorylation
S398 Phosphorylation
C453 S-Nitrosylation
K486 Ubiquitination
S496 Phosphorylation
Y509 Phosphorylation
K521 Ubiquitination
K522 Ubiquitination
S528 Phosphorylation
K530 Ubiquitination
S534 Phosphorylation
Y536 Phosphorylation P06213 (INSR) , P00519 (ABL1) , P12931 (SRC) , A0A173G4P4 (Abl fusion) , P06239 (LCK) , P07948 (LYN)
T540 Phosphorylation
Y541 Phosphorylation
K546 Ubiquitination
S553 Phosphorylation
T555 Phosphorylation
S556 Phosphorylation
S557 Phosphorylation
K560 Ubiquitination
Y564 Phosphorylation A0A173G4P4 (Abl fusion) , P00519 (ABL1) , P07948 (LYN) , P06239 (LCK) , P12931 (SRC)
K570 Ubiquitination
S582 Phosphorylation
S588 Phosphorylation
S591 Phosphorylation P17252 (PRKCA) , P24941 (CDK2)

研究背景

功能:

Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

翻译修饰:

Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.

细胞定位:

Cytoplasm. Nucleus.
Note: In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.

亚基结构:

Monomer. Interacts with MTUS1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Interacts with KIT (By similarity). Interacts with SIRPA/PTPNS1. Interacts with LILRB1 and LILRB2. Interacts with FCRL2 and FCRL4. Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form). Interacts with CD84. Interacts with CD300LF. Interacts with CDK2. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with moesin/MSN.

蛋白家族:

The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation.

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

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