产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12190, RRID:AB_2844995.
展开/折叠
63 kDa membrane protein; CKAP 4; Ckap4; CKAP4_HUMAN; Cytoskeleton associated protein 4; Cytoskeleton-associated protein 4; ERGIC 63; ERGIC63; MGC99554; p63; Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment; Type II transmembrane protein p63;
抗原和靶标
- Q07065 CKAP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSAKQRGSKGGHGAASPSEKGAHPSGGADDVAKKPPPAPQQPPPPPAPHPQQHPQQHPQNQAHGKGGHRGGGGGGGKSSSSSSASAAAAAAAASSSASCSRRLGRALNFLFYLALVAAAAFSGWCVHHVLEEVQQVRRSHQDFSRQREELGQGLQGVEQKVQSLQATFGTFESILRSSQHKQDLTEKAVKQGESEVSRISEVLQKLQNEILKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFEALQQKSQGLDSRLQHVEDGVLSMQVASARQTESLESLLSKSQEHEQRLAALQGRLEGLGSSEADQDGLASTVRSLGETQLVLYGDVEELKRSVGELPSTVESLQKVQEQVHTLLSQDQAQAARLPPQDFLDRLSSLDNLKASVSQVEADLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLRNDLDRLFVKVEKIHEKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q07065 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| C100 | S-Nitrosylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K270 | Acetylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| Y322 | Phosphorylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| T512 | O-Glycosylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| K551 | Methylation | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| Y563 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| R590 | Methylation | Uniprot |
研究背景
High-affinity epithelial cell surface receptor for APF.
Mediates the anchoring of the endoplasmic reticulum to microtubules.
Reversibly palmitoylated. Palmitoylation at Cys-100 by DHHC2 is required for its trafficking from the ER to the plasma membrane and for its perinuclear localization.
Increased phosphorylation during mitosis prevents binding to microtubules.
Endoplasmic reticulum membrane>Single-pass type II membrane protein. Cell membrane>Single-pass type II membrane protein. Cytoplasm>Cytoskeleton. Cytoplasm>Perinuclear region.
Note: Translocates to the perinuclear region upon APF-stimulation.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
限制条款
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