| 产品: | DNAJC10 抗体 |
| 货号: | DF12269 |
| 描述: | Rabbit polyclonal antibody to DNAJC10 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 75-80 kDa; 91kD(Calculated). |
| 蛋白号: | Q8IXB1 |
| RRID: | AB_2845074 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12269, RRID:AB_2845074.
展开/折叠
DKFZp434J1813; DnaJ (Hsp40) homolog subfamily C member 10; DnaJ homolog subfamily C member 10; DNAJC 10; ER resident protein ERdj5; ERdj 5; ERdj5; J domain containing protein disulfide isomerase like protein; JPDI; Macrothioredoxin; MGC104194; MTHr;
抗原和靶标
- Q8IXB1 DJC10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVWLNKDDYIRDLKRIILCFLIVYMAILVGTDQDFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNNPNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWNYYRYDFGIYDDDPEIITLERREFDAAVNSGELWFVNFYSPGCSHCHDLAPTWRDFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSGMAPVKYHGDRSKESLVSFAMQHVRSTVTELWTGNFVNSIQTAFAAGIGWLITFCSKGGDCLTSQTRLRLSGMLDGLVNVGWMDCATQDNLCKSLDITTSTTAYFPPGATLNNKEKNSILFLNSLDAKEIYLEVIHNLPDFELLSANTLEDRLAHHRWLLFFHFGKNENSNDPELKKLKTLLKNDHIQVGRFDCSSAPDICSNLYVFQPSLAVFKGQGTKEYEIHHGKKILYDILAFAKESVNSHVTTLGPQNFPANDKEPWLVDFFAPWCPPCRALLPELRRASNLLYGQLKFGTLDCTVHEGLCNMYNIQAYPTTVVFNQSNIHEYEGHHSAEQILEFIEDLMNPSVVSLTPTTFNELVTQRKHNEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSIDCQQYHSFCAQENVQRYPEIRFFPPKSNKAYHYHSYNGWNRDAYSLRIWGLGFLPQVSTDLTPQTFSEKVLQGKNHWVIDFYAPWCGPCQNFAPEFELLARMIKGKVKAGKVDCQAYAQTCQKAGIRAYPTVKFYFYERAKRNFQEEQINTRDAKAIAALISEKLETLRNQGKRNKDEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IXB1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K66 | Ubiquitination | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K640 | Acetylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| T673 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| K778 | Ubiquitination | Uniprot | |
| R783 | Methylation | Uniprot |
研究背景
Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.
Endoplasmic reticulum lumen.
Interacts with EDEM1 (By similarity). Interacts with HSPA5 (via its J domain).
The thioredoxin-like regions Trxb 1 and 2 lack a redox-active CXXC motif.
Thioredoxin domains 3 and 4 are the primary reductase domains.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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