产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12449, RRID:AB_2845254.
展开/折叠
Endoplasmic reticulum protein 5; ER protein 5; ERp5; P5; Pdia6; PDIA6_HUMAN; Protein disulfide isomerase A6; Protein disulfide isomerase associated 6; Protein disulfide isomerase family A member 6; Protein disulfide isomerase P5; Protein disulfide isomerase related protein; Protein disulfide-isomerase A6; Thioredoxin domain containing 7 (protein disulfide isomerase); Thioredoxin domain containing protein 7; Thioredoxin domain-containing protein 7; TXNDC7;
抗原和靶标
- Q15084 PDIA6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALLVLGLVSCTFFLAVNGLYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15084 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S22 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K66 | Methylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K77 | Ubiquitination | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| R142 | Methylation | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| K241 | Acetylation | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| Y252 | Phosphorylation | Uniprot | |
| S270 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| Y316 | Phosphorylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| R392 | Methylation | Uniprot | |
| R409 | Methylation | Uniprot | |
| S428 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
研究背景
May function as a chaperone that inhibits aggregation of misfolded proteins. Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling. May also regulate the UPR via the EIF2AK3 UPR sensor. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.
Endoplasmic reticulum lumen. Cell membrane. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).
Expressed in platelets (at protein level).
Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with MICA on the surface of tumor cells, leading to MICA disulfide bond reduction which is required for its release from tumor cells. Interacts with ITGB3 following platelet stimulation. Interacts with ERN1; the interaction is direct. Interacts with EIF2AK3.
Belongs to the protein disulfide isomerase family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
限制条款
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