产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12494, RRID:AB_2845299.
展开/折叠
UB fusion protein 1; Ubiquitin fusion degradation 1 like (yeast); Ubiquitin fusion degradation 1 like; Ubiquitin fusion degradation protein 1 homolog; UFD1; UFD1_HUMAN; UFD1L;
抗原和靶标
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
- Q92890 UFD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERQVQHEESTEGEADHSGYAGELGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFVAFSGEGQSLRKKGRKP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92890 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| T78 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| R170 | Methylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S231 | Phosphorylation | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K280 | Ubiquitination | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| K302 | Ubiquitination | Uniprot |
研究背景
Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I.
Nucleus. Cytoplasm>Cytosol.
Found in adult heart, skeletal muscle and pancreas, and in fetal liver and kidney.
Heterodimer with NPLOC4, this heterodimer binds VCP and inhibits Golgi membrane fusion. Interacts with USP13. Interacts with ZFAND2B; probably through VCP.
Belongs to the UFD1 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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