产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12598, RRID:AB_2845560.
展开/折叠
DJ10; DJB12_HUMAN; DKFZp586B2023; DnaJ (Hsp40) homolog, subfamily B, member 12; DnaJ homolog subfamily B member 12; Dnajb12;
抗原和靶标
- Q9NXW2 DJB12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVRALIESLNQKPQTAGDQPPPTDTTHATHRKAGGTDAPSANGEAGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRGASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHRRVTDHLGVVYYVGDTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDTDMYHRAQKMGTPSCSRLSEVQASLHG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NXW2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| Y158 | Phosphorylation | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| Y321 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| Y345 | Phosphorylation | Uniprot | |
| Y352 | Phosphorylation | Uniprot |
研究背景
Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.
(Microbial infection) In case of infection by polyomavirus, involved in the virus endoplasmic reticulum membrane penetration and infection.
Endoplasmic reticulum membrane>Single-pass membrane protein. Nucleus membrane>Single-pass membrane protein.
Note: Localizes to the endoplasmic reticulum membrane (PubMed:21150129, PubMed:21148293, PubMed:24732912, PubMed:27916661). When overexpressed, forms membranous structures in the nucleus (PubMed:24732912).
Homodimer and homotetramer. Interacts (via J domain) with HSPA8/Hsc70. Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct.
Belongs to the DnaJ family. DNAJB12/DNAJB14 subfamily.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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