| 产品: | NSFL1C 抗体 |
| 货号: | DF12677 |
| 描述: | Rabbit polyclonal antibody to NSFL1C |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 41 kDa; 41kD(Calculated). |
| 蛋白号: | Q9UNZ2 |
| RRID: | AB_2845639 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12677, RRID:AB_2845639.
展开/折叠
DJ776F14.1; MGC3347; NSF1C_HUMAN; NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; NSFL1C; OTTHUMP00000029972; OTTHUMP00000029974; p47; P47 protein; p97 cofactor p47; SHP1 homolog; UBX domain protein 2C; UBX domain-containing protein 2C; UBX1; UBXD10; UBXN2C;
抗原和靶标
- Q9UNZ2 NSF1C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UNZ2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S68 | Phosphorylation | Uniprot | |
| T73 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| R93 | Methylation | Uniprot | |
| Y95 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K124 | Methylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K146 | Acetylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| K172 | Acetylation | Uniprot | |
| K172 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| R199 | Methylation | Uniprot | |
| R223 | Methylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K247 | Methylation | Uniprot | |
| K247 | Ubiquitination | Uniprot | |
| K251 | Acetylation | Uniprot | |
| K251 | Methylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| S271 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| K356 | Ubiquitination | Uniprot |
研究背景
Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis.
Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis (By similarity).
Nucleus. Golgi apparatus>Golgi stack. Chromosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Predominantly nuclear in interphase cells. Bound to the axial elements of sex chromosomes in pachytene spermatocytes. A small proportion of the protein is cytoplasmic, associated with Golgi stacks. Localizes to centrosome during mitotic prophase and metaphase.
Part of a ternary complex containing STX5A, NSFL1C and VCP. NSFL1C forms a homotrimer that binds to one end of a VCP homohexamer. The complex binds to membranes enriched in phosphatidylethanolamine-containing lipids and promotes Golgi membrane fusion. Interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP. Binds ubiquitin and mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP (By similarity).
Belongs to the NSFL1C family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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