| 产品: | SEC13 抗体 |
| 货号: | DF12734 |
| 描述: | Rabbit polyclonal antibody to SEC13 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 36 kDa; 36kD(Calculated). |
| 蛋白号: | P55735 |
| RRID: | AB_2845695 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12734, RRID:AB_2845695.
展开/折叠
D3S1231E; Npp 20; Npp20; Protein SEC13 homolog; SEC13 (S. cerevisiae) like 1; Sec13; SEC13 homolog (S. cerevisiae); SEC13 homolog; SEC13 like 1 (S. cerevisiae); SEC13 like 1 isoform; SEC13 like protein 1; SEC13 protein; SEC13 related protein; SEC13-like protein 1; SEC13-related protein; SEC13_HUMAN; SEC13R;
抗原和靶标
- P55735 SEC13_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSVINTVDTSHEDMIHDAQMDYYGTRLATCSSDRSVKIFDVRNGGQILIADLRGHEGPVWQVAWAHPMYGNILASCSYDRKVIIWREENGTWEKSHEHAGHDSSVNSVCWAPHDYGLILACGSSDGAISLLTYTGEGQWEVKKINNAHTIGCNAVSWAPAVVPGSLIDHPSGQKPNYIKRFASGGCDNLIKLWKEEEDGQWKEEQKLEAHSDWVRDVAWAPSIGLPTSTIASCSQDGRVFIWTCDDASSNTWSPKLLHKFNDVVWHVSWSITANILAVSGGDNKVTLWKESVDGQWVCISDVNKGQGSVSASVTEGQQNEQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P55735 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| V2 | Acetylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| R35 | Methylation | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| T92 | Phosphorylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot |
研究背景
Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Required for the exit of adipsin (CFD/ADN), an adipocyte-secreted protein from the endoplasmic reticulum (By similarity).
As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1.
Cytoplasmic vesicle>COPII-coated vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Nucleus>Nuclear pore complex. Lysosome membrane.
Note: In interphase, localizes at both sides of the NPC.
At the nuclear pore: component of the Y-shaped Nup107-160 subcomplex of the nuclear pore complex (NPC). The Nup107-160 subcomplex includes NUP160, NUP133, NUP107, NUP98, NUP85, NUP43, NUP37, SEH1 and SEC13. At the COPII coat complex: interacts with SEC31A and SEC31B. Within the GATOR complex, component of the GATOR2 subcomplex, made of MIOS, SEC13, SEH1L, WDR24 and WDR59. The GATOR complex strongly interacts with RRAGA/RRAGC and RRAGB/RRAGC heterodimers. The GATOR2 complex interacts with CASTOR2 and CASTOR1; the interaction is negatively regulated by arginine. The GATOR2 complex interacts with SESN1, SESN2 and SESN3; the interaction is negatively regulated by amino acids. Interacts with SEC16A. Interacts with SEC16B.
Belongs to the WD repeat SEC13 family.
研究领域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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