| 产品: | NPLOC4 抗体 |
| 货号: | DF13194 |
| 描述: | Rabbit polyclonal antibody to NPLOC4 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 68 kDa; 68kD(Calculated). |
| 蛋白号: | Q8TAT6 |
| RRID: | AB_2846154 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13194, RRID:AB_2846154.
展开/折叠
KIAA1499; NPL4; NPL4, S. cerevisiae, homolog of; NPL4_HUMAN; nploc4; nuclear protein localization 4 homolog (S. cerevisiae); Nuclear protein localization 4 homolog; Nuclear protein localization protein 4 homolog; Nuclear protein localization protein 4, S. cerevisiae, homolog of; Protein NPL4;
抗原和靶标
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
- Q8TAT6 NPL4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSLAGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TAT6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| T28 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| T172 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| C188 | S-Nitrosylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| K544 | Ubiquitination | Uniprot |
研究背景
The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I.
Cytoplasm>Cytosol. Endoplasmic reticulum. Nucleus.
Note: Associated with the endoplasmic reticulum and nuclear.
Expressed at highest levels in brain, heart, skeletal muscle, kidney and fetal liver.
Heterodimer with UFD1. The heterodimer binds ubiquitinated proteins (By similarity). The heterodimer binds to VCP and inhibits Golgi membrane fusion. Interacts with ZFAND2B; probably through VCP.
Binds ubiquitinated proteins via its RanBP2-type zinc finger.
Belongs to the NPL4 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.