Cleaved-Caspase 3 (Asp175), p17 Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of extracts from various samples, using Actived Caspase 3 mouse monoclonal antibody.

Figure 5 DTX-PM improve DTX efficacy in a PDX model of GI cancer.

Figure 2.

Fig.

Figure 7 NUPR1 inhibition induced cell death by apoptosis in KrasG12D expressing cells in vivo.

产品:	Cleaved-Caspase 3 (Asp175), p17 抗体
货号:	BF0711
描述:	Mouse monoclonal antibody to Cleaved-Caspase 3 (Asp175), p17
应用:	WB IHC IF/ICC
反应:	Human, Mouse, Rat
分子量:	17kDa; 32kD(Calculated).
蛋白号:	P42574
RRID:	AB_2846190

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实验方案

产品描述

来源:

Mouse

应用:

WB(1:500-1:2000), IHC(1:200-1:1000), IF(1:50-1:200)
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

克隆:

Monoclonal [AFB17776]

特异性:

Caspase 3 (p17,Cleaved-Asp175) Antibody detects endogenous levels of fragment of activated Caspase 3 resulting from cleavage adjacent to Asp175.

RRID:

AB_2846190
引用格式: Affinity Biosciences Cat# BF0711, RRID:AB_2846190.

偶联:

Unconjugated.

纯化:

Affinity-chromatography.

保存:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

A830040C14Rik; Apopain; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3, apoptosis-related cysteine peptidase; Caspase 3, apoptosis-related cysteine protease; Caspase 3, apoptosis-related cysteine protease a; Caspase-3 subunit p12; CC3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; protein Yama; SCA 1; SCA-1; SREBP cleavage activity 1; Yama;

抗原和靶标

免疫原:

Purified recombinant fragment of human Caspase 3 expressed in E. Coli.

Uniprot:

P42574(CASP3_HUMAN) >>Visit HPA database.

基因/基因ID:

CASP3(836)

表达:

P42574 CASP3_HUMAN:

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

序列:

P42574 CASP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENTENSVDSKSIKNLEPKIIHGSESMDSGISLDNSYKMDYPEMGLCIIINNKNFHKSTGMTSRSGTDVDAANLRETFRNLKYEVRNKNDLTREEIVELMRDVSKEDHSKRSSFVCVLLSHGEEGIIFGTNGPVDLKKITNFFRGDRCRSLTGKPKLFIIQACRGTELDCGIETDSGVDDDMACHKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCAMLKQYADKLEFMHILTRVNRKVATEFESFSFDATFHAKKQIPCIVSMLTKELYFYH

翻译修饰 - P42574 作为底物

P42574

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
T4	Phosphorylation		Uniprot
S7	Phosphorylation		Uniprot
S10	Phosphorylation		Uniprot
K11	Acetylation		Uniprot
K11	Ubiquitination		Uniprot
S12	Phosphorylation		Uniprot
K14	Ubiquitination		Uniprot
K19	Ubiquitination		Uniprot
S24	Phosphorylation		Uniprot
S26	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
Y41	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
S65	Phosphorylation		Uniprot
T67	Phosphorylation		Uniprot
K82	Acetylation		Uniprot
K82	Ubiquitination		Uniprot
K88	Ubiquitination		Uniprot
K105	Ubiquitination		Uniprot
K138	Ubiquitination		Uniprot
S150	Phosphorylation	Q16539 (MAPK14)	Uniprot
T152	Phosphorylation		Uniprot
C163	S-Nitrosylation		Uniprot
T174	Phosphorylation		Uniprot
S176	Phosphorylation		Uniprot
K210	Ubiquitination		Uniprot
K229	Ubiquitination		Uniprot
S249	Phosphorylation		Uniprot
K260	Ubiquitination		Uniprot
T270	Phosphorylation		Uniprot

研究背景

P42574_CASP3_HUMAN

功能:

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

翻译修饰:

Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

细胞定位:

Cytoplasm.

组织特异性:

亚基结构:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.

蛋白家族:

Belongs to the peptidase C14A family.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Nervous system > Serotonergic synapse.

文献引用

1). Π electron-stabilized polymeric micelles potentiate docetaxel therapy in advanced-stage gastrointestinal cancer. BIOMATERIALS, 2021 (PubMed: 33069116) [IF=14.0]

Application: IF/ICC Species: Mice Sample: Tumor

Figure 5 DTX-PM improve DTX efficacy in a PDX model of GI cancer. A: Tumor growth inhibition upon treatment with PBS, and with 10 mg/kg of free DTX and DTX-PM. B: Tumor weights at the end of the experiment, showing significantly more efficient tumor inhibition for DTX-PM than for free DTX. C-E: Immunofluorescence analysis of cellular proliferation (Ki67) and apoptosis induction (cleaved caspase 3), confirming that DTX-PM was more effective than free DTX. F and G: Individual PDX tumor growth curves in the mice treated with DTX and DTX-PM as compared to PBS controls, exemplifying the superior efficacy of DTX-PM. H and I: PDX tumors were analyzed by means of immunofluorescence microscopy, showing a substantially increased ratio of M1-like to M2-like TAM upon DTX-PM treatment.

2). Targeting NUPR1-dependent stress granules formation to induce synthetic lethality in KrasG12D-driven tumors. EMBO molecular medicine, 2024 (PubMed: 38360999) [IF=11.1]

Application: IF/ICC Species: Mouse Sample:

Figure 7 NUPR1 inhibition induced cell death by apoptosis in KrasG12D expressing cells in vivo. Immunohistofluorescence staining was performed on histologic sections of the pancreas of the different experimental groups. Rabbit anti-KrasG12D primary antibody and mouse anti-cleaved caspase 3 antibody was used. DNA was stained with DAPI. Pearson coefficient between both channels was calculated by JACoP, ImageJ. Data represent mean ± SD, ANOVA with Sidak correction (n = 5 mice).

3). Oral P. gingivalis impairs gut permeability and mediates immune responses associated with neurodegeneration in LRRK2 R1441G mice. Journal of Neuroinflammation, 2020 (PubMed: 33213462) [IF=9.3]

Application: WB Species: mice Sample: R1441G cells and FVBN cells

Fig.1 c Representative images of western blots of cleaved caspase-3 protein levels and quantitative analysis, which were done with the SN obtained from F + Pg and 1441 + Pg mice compared to F + C and 1441 + C mice. n = 4, **P < 0.01. Two-way ANOVA and Tukey’s test were used for analysis.

4). Smooth muscle-specific HuR knockout induces defective autophagy and atherosclerosis. Cell Death & Disease, 2021 (PubMed: 33837179) [IF=9.0]

Application: WB Species: mouse Sample: SMCs

Fig. 3| Loss of HuR promoted apoptosis in atherosclerosis. A Immunofluorescent staining of aortic roots from CTR and HuRSMKO mice to determine TUNEL-positive VSMCs. Red puncta denotes TUNEL-positive cells. Green region denotes α-SMA. Scale bar = 20 μm. B Immunohistochemical staining of cleaved caspase-3 in aortic roots (n = 6). Scale bar = 50 μm. C Serum lipid profiles (total cholesterol [TC], triglycerides [TG], high-density lipoprotein cholesterol [HDL-C], and low-density lipoprotein cholesterol [LDL-C]) (n = 6). D Western blot analysis of cleaved caspase-3 in control and HuR-deficient SMCs (n = 5).

5). Histone deacetylase-mediated silencing of PSTPIP2 expression contributes to aristolochic acid nephropathy-induced PANoptosis. British journal of pharmacology, 2024 (PubMed: 38073114) [IF=7.3]

6). Focal ischemic stroke modifies microglia-derived exosomal miRNAs: potential role of mir-212-5p in neuronal protection and functional recovery. Biological Research, 2023 (PubMed: 37789455) [IF=6.7]

Application: IF/ICC Species: Rat Sample: PC12 cells

Fig. 9 MiR-212-5p reduces neuronal apoptosis and attenuates axonal degeneration in PC12 cells following OGD/R. A Cleaved-Caspase 3 immunofluorescence staining (in green). Nuclei were stained with DAPI and are visualised in blue. Scale bar = 50 μm. B, C Nogo-A and NgR gene expression levels in PC12 cells. Gene expression levels were examined using qRT–PCR and normalised to β-actin. D-F Immunofluorescence staining was conducted to reveal the presence of Nogo-A, NgR and β III tubulin in PC12 cells. Nuclei were stained with DAPI and are visualised in blue. Scale bar = 50 μm. The data are presented as the means ± SEM (n = 5 per group). *P

7). PLXNA2 knockdown promotes M2 microglia polarization through mTOR/STAT3 signaling to improve functional recovery in rats after cerebral ischemia/reperfusion injury. EXPERIMENTAL NEUROLOGY, 2021 (PubMed: 34474008) [IF=5.3]

Application: IF/ICC Species: Mice Sample: BV2 microglia cells

Fig. 8. The effects of PLXNA2 knockdown on the mTOR/STAT3 pathway in oxygen and glucose deprivation/reoxygenation (OGD/R)-stimulated BV2 microglia cells. (A) Immunofluorescence staining for PLXNA2 in OGD/R-stimulated BV2 microglia cells. Scale bar = 50 μm. (B) Immunofluorescence staining for cleaved caspase 3 in OGD/R-stimulated BV2 microglia cells. Scale bar = 50 μm. (C) Representative Western blot bands show the protein expression of PLXNA2, p-mTOR, mTOR, p-STAT3 and STAT3 in BV2 microglia cells. β-actin was used as internal control. (D–F) Quantification of immunoblots of PLXNA2, p-mTOR and p-STAT3. The data are presented as the mean ± SEM for each group (n = 4–6 per group). *p < 0.05, **p < 0.01 versus the sham + scrambled group, #p < 0.05, ##p < 0.01 versus the MCAO/ R + scrambled group.

8). Fibroblast growth factor 2 contributes to the effect of salidroside on dendritic and synaptic plasticity after cerebral ischemia/reperfusion injury. Aging (Albany NY), 2020 (PubMed: 32518214) [IF=5.2]

Application: IF/ICC Species: rat Sample:

Application: WB Species: rat Sample:

Figure 8.| Sal attenuates neuronal apoptosis after MCAO/R. (A) Immunofluorescence staining of c-caspase 3 in sections from the ischemic penumbra in each group on day 7 post-MCAO/R (the scale bar is 20 μm).. (B–E) Protein expression of c-caspase 3, Bcl-2 and Bax from the ischemic penumbra. (F–H) QPCR results of c-caspase 3, Bcl-2 and Bax expression. Values are expressed as the mean ± SD. #p < 0.05, ##p <0.01 vs. sham; *p < 0.05, **p < 0.01 vs. MCAO/R.

9). Tannic acid alleviates lipopolysaccharide‑induced H9C2 cell apoptosis by suppressing reactive oxygen species‑mediated endoplasmic reticulum stress. Molecular Medicine Reports, 2021 (PubMed: 34080663) [IF=3.4]

Application: WB Species: Rat Sample: H9C2 cells

Figure 2. TA alleviates the LPS-induced apoptosis of H9C2 cells. (A) Flow cytometry was used to determine cell apoptotic rate using Annexin V-FITC/PI staining. Apoptotic cells included Annexin V+/PI- and Annexin V+/PI+ cells. (B) Quantitative analysis of the apoptotic rate of cells treated with LPS, TA and TA + LPS. (C) Protein expression levels of Bax, Bcl-2, cleaved caspase-9, cleaved caspase-3 and β-actin in H9C2 cells treated with LPS, TA or TA + LPS were determined by western blot analysis. (D) Semi-quantification of the protein expression levels of Bax/Bcl-2, cleaved caspase-9 and cleaved caspase-3 in the LPS, TA and TA + LPS groups. Data are presented as the mean ± standard error of the mean (n=3). *P

10). Tannic Acid Alleviates Lipopolysaccharide-Induced H9C2 Cell Apoptosis by Suppressing ROS-Mediated ER Stress. Molecular Medicine Reports, 2021 (PubMed: 34080663) [IF=3.4]

Application: WB Species: rat Sample: H9C2 cells

Figure 2.| TA alleviates the LPS‑induced apoptosis of H9C2 cells. (C) Protein expression levels of Bax, Bcl‑2, cleaved caspase‑9, cleaved caspase‑3 and β‑actin in H9C2 cells treated with LPS, TA or TA + LPS were determined by western blot analysis.

Application: WB Species: Rat Sample: H9C2 cells

Figure 6. TA attenuates LPS-induced apoptosis via suppressing ROS-mediated endoplasmic reticulum stress in H9C2 cells. (A) Whole cell lysates were prepared following stimulation with LPS, NAC and TA. The protein expression levels of apoptosis-associated proteins (p-JNK, JNK, Bax, cleaved caspase-9 and cleaved caspase-3) and an anti-apoptotic protein (Bcl-2) were detected in H9C2 cells treated with LPS, NAC + LPS, TA + LPS and TA + NAC + LPS by western blot analysis. Semi-quantification of the protein expression levels of (B) p-JNK/JNK and cleaved caspase-3, and (C) Bax/Bcl-2 and cleaved caspase-9 in H9C2 cells. The mRNA expression levels of (D) Bax, (E) caspase-3, (F) caspase-12 and (G) Cyt c in H9C2 cells normalized to Gapdh. Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 and ***P<0.001 vs. control; #P<0.05 and ##P<0.01 vs. LPS group; §P<0.05 and §§P<0.01 vs. TA + LPS. TA, tannic acid; LPS, lipopolysaccharide; NAC, N-acetylcysteine; p, phosphorylated; JNK, c-Jun N-terminal kinase; Cyt c, cytochrome c.

Application: WB Species: Rat Sample: H9C2 cells

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Cleaved-Caspase 3 (Asp175), p17 Antibody - #BF0711

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产品描述

抗原和靶标

翻译修饰 - P42574 作为底物

研究背景

研究领域

文献引用

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