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Western blot analysis of extracts from various samples, using Cytochrome C mouse monoclonal antibody.

产品:	Cytochrome C 抗体
货号:	BF0714
描述:	Mouse monoclonal antibody to Cytochrome C
应用:	WB IHC
反应:	Human, Mouse, Rat, Rabbit
分子量:	11kDa; 12kD(Calculated).
蛋白号:	P99999
RRID:	AB_2846192

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实验方案

产品描述

来源:

Mouse

应用:

WB(1:500-1:2000), IHC(1:100-1:1000)
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat,Rabbit

克隆:

Monoclonal [AFB17778]

特异性:

Cytochrome C antibody detects endogenous levels of total Cytochrome C.

RRID:

AB_2846192
引用格式: Affinity Biosciences Cat# BF0714, RRID:AB_2846192.

偶联:

Unconjugated.

纯化:

Affinity-chromatography.

保存:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

CYC; CYC_HUMAN; CYCS; Cytochrome c; Cytochrome c somatic; HCS; THC4;

抗原和靶标

免疫原:

Purified recombinant fragment of human Cytochrome C expressed in E. Coli.

Uniprot:

P99999(CYC_HUMAN) >>Visit HPA database.

基因/基因ID:

CYCS(54205)

序列:

P99999 CYC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

翻译修饰 - P99999 作为底物

P99999

Site	PTM Type	Source
G2	Acetylation	Uniprot
K9	Acetylation	Uniprot
K28	Sumoylation	Uniprot
K28	Ubiquitination	Uniprot
T29	Phosphorylation	Uniprot
K40	Ubiquitination	Uniprot
T41	Phosphorylation	Uniprot
Y47	Phosphorylation	Uniprot
S48	Phosphorylation	Uniprot
Y49	Phosphorylation	Uniprot
T50	Phosphorylation	Uniprot
K54	Ubiquitination	Uniprot
Y68	Phosphorylation	Uniprot
K73	Acetylation	Uniprot
K73	Ubiquitination	Uniprot
K74	Acetylation	Uniprot
K74	Ubiquitination	Uniprot
Y75	Phosphorylation	Uniprot
T79	Phosphorylation	Uniprot
K80	Ubiquitination	Uniprot
K87	Acetylation	Uniprot
K87	Ubiquitination	Uniprot
K88	Acetylation	Uniprot
K89	Acetylation	Uniprot
Y98	Phosphorylation	Uniprot
K100	Acetylation	Uniprot
K100	Methylation	Uniprot
K100	Ubiquitination	Uniprot
K101	Methylation	Uniprot

研究背景

P99999_CYC_HUMAN

功能:

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

翻译修饰:

Binds 1 heme group per subunit.

Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

细胞定位:

Mitochondrion intermembrane space.
Note: Loosely associated with the inner membrane.

蛋白家族:

Belongs to the cytochrome c family.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Metabolism > Energy metabolism > Sulfur metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Design, synthesis and biological evaluation of N-anthraniloyl tryptamine derivatives as pleiotropic molecules for the therapy of malignant glioma. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 34091208) [IF=6.7]

Application: WB Species: Human Sample: U251 and U87MG cells.

Fig. 7. NP16 induces U87MG and U251 apoptosis and ROS generation at various concentrations (1, 2 and 4 mM). (A, B): images of apoptosis-related key protein (Bax, Bcl-2 and cytochrome c) expression levels of detected by western blotting method in U251 and U87MG cells. b-actin was used as an internal loading control. (CeF) Quantification of Bcl-2/Bax ratio and cytochrome c using ImageJ software. (G) Representative DCFH-DA fluorescence images of U251 and U87MG cells treated by NP16 at 1, 2 and 4 mM (magnification, 10, scale bar ¼ 500 mm). (HeI) Reactive ROS generation ratio qualified by DCFH-DA fluorescence density in U251 and U87MG using image J. All data are expressed as the mean ± SD pooled from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 when compared with the DMSO groups treated by equivalent amounts of DMSO.

2). Melatonin derivatives combat with inflammation-related cancer by targeting the Main Culprit STAT3. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 33248852) [IF=6.7]

Application: WB Species: human Sample: HCT-116 cells

Figure 10. (A) Compound P-3 triggered the explosion of ROS in HCT-116 cells. (magnification 20 ×, scale bar = 100 μm) (B) P-3 regulated the expression of proteins involved in Cyto-c/Caspase3 pathway. Data are presented as mean ± SD from three independent experiments. * P < 0.05, ** P<0.01, *** P<0.001 versus the control group.

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