DFFB Antibody - #DF13360

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产品: DFFB 抗体
货号: DF13360
描述: Rabbit polyclonal antibody to DFFB
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
分子量: 39kDa; 39kD(Calculated).
蛋白号: O76075
RRID: AB_2846379

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(88%), Zebrafish(83%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
克隆:
Polyclonal
特异性:
DFFB Antibody detects endogenous levels of total DFFB.
RRID:
AB_2846379
引用格式: Affinity Biosciences Cat# DF13360, RRID:AB_2846379.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CAD; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; Caspase-activated nuclease; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF-40; DFF2; DFF40; Dffb; DFFB_HUMAN; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40 kD beta subunit; DNA fragmentation factor, 40 kD, beta polypeptide; DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase); OTTHUMP00000003633;

抗原和靶标

免疫原:
Uniprot:

O76075(DFFB_HUMAN) >>Visit HPA database.

基因/基因ID:
DFFB(1677)
序列:
MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRKRQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Zebrafish
83
Xenopus
75
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O76075 作为底物

Site PTM Type Enzyme
K152 Ubiquitination
Y158 Phosphorylation
K264 Ubiquitination
K265 Ubiquitination
K277 Ubiquitination
S319 Phosphorylation
Y322 Phosphorylation
K323 Ubiquitination
T326 Phosphorylation

研究背景

功能:

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Heterodimer of DFFA and DFFB. Interacts with H1-1.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

文献引用

1). Downregulation of the Long Noncoding RNA IALNCR Targeting MAPK8/JNK1 Promotes Apoptosis and Antagonizes Bovine Viral Diarrhea Virus Replication in Host Cells. Journal of Virology, 2022 (PubMed: 35993735) [IF=4.0]

Application: WB    Species: Bovine    Sample: MDBK cells and BT cell

FIG 3 mRNA levels of apoptosis-related genes caspase-3 and Bcl-2 (A), and Bax and Bak1 (B) detected by qRT-PCR at different time points after BVDV infection (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (C) Protein levels of caspase-3 and Bcl-2 were detected by Western blotting at different time points after BVDV infection. Lowercase letters (a versus b; b versus c) indicate a significant difference of P < 0.05; a versus c indicates a significant difference of P < 0.01. (D) Protein levels of apoptosis-related pathway proteins were detected by Western blotting (***, P < 0.001). (E) Identification of procaspase-3/8/9 cleavage, ICAD/DFF45 degradation, nuclear translocation of active caspase-3 and CAD/DFF40, and nuclear PARP cleavage by Western blotting in BVDV-infected MDBK cells and BT cells at different time points after infection.

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