产品: hFANCI 抗体
货号: AF6524
描述: Rabbit polyclonal antibody to hFANCI
应用: ELISA(peptide)
反应: Human
分子量: 149kD(Calculated).
蛋白号: Q9NVI1
RRID: AB_2847248

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产品描述

来源:
Rabbit
应用:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
hFANCI Antibody detects endogenous levels of total hFANCI.
RRID:
AB_2847248
引用格式: Affinity Biosciences Cat# AF6524, RRID:AB_2847248.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FANCI; FANCI gene; FANCI_HUMAN; Fanconi anemia group I protein; Fanconi anemia, complementation group I; FLJ10719; FLJ14658; KIAA1794; Protein FACI; Protein FANCI;

抗原和靶标

免疫原:

A synthesized peptide derived from human hFANCI.

Uniprot:
基因/基因ID:
序列:
MDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMDSLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK

翻译修饰 - Q9NVI1 作为底物

Site PTM Type Enzyme
K4 Ubiquitination
K12 Ubiquitination
K16 Acetylation
K16 Ubiquitination
K39 Ubiquitination
K41 Ubiquitination
K52 Ubiquitination
T62 Phosphorylation
K125 Ubiquitination
K141 Ubiquitination
K148 Ubiquitination
S152 Phosphorylation
K157 Ubiquitination
K158 Ubiquitination
Y215 Phosphorylation
S222 Phosphorylation
K331 Ubiquitination
K336 Ubiquitination
K339 Ubiquitination
K348 Ubiquitination
K402 Ubiquitination
T406 Phosphorylation
S407 Phosphorylation
S409 Phosphorylation
S411 Phosphorylation
K420 Ubiquitination
K431 Ubiquitination
K501 Ubiquitination
K523 Ubiquitination
S556 Phosphorylation Q13315 (ATM)
S559 Phosphorylation Q13315 (ATM)
S565 Phosphorylation
Y606 Phosphorylation
Y610 Phosphorylation
S617 Phosphorylation
K638 Ubiquitination
K646 Acetylation
T653 Phosphorylation
K715 Ubiquitination
K725 Ubiquitination
S726 Phosphorylation
S730 Phosphorylation Q13315 (ATM)
K780 Ubiquitination
S782 Phosphorylation
K788 Ubiquitination
K799 Ubiquitination
T800 Phosphorylation
K849 Ubiquitination
K854 Ubiquitination
K869 Ubiquitination
K897 Ubiquitination
K902 Ubiquitination
S903 Phosphorylation
K926 Ubiquitination
K939 Ubiquitination
T952 Phosphorylation
S972 Phosphorylation Q13315 (ATM)
S1000 Phosphorylation
K1015 Ubiquitination
S1027 Phosphorylation
K1040 Ubiquitination
K1068 Ubiquitination
K1105 Ubiquitination
K1107 Ubiquitination
S1120 Phosphorylation
S1121 Phosphorylation Q13315 (ATM)
K1196 Ubiquitination
K1199 Ubiquitination
K1221 Ubiquitination
K1229 Ubiquitination
K1232 Ubiquitination
T1238 Phosphorylation
K1248 Ubiquitination
K1272 Ubiquitination
K1280 Ubiquitination
K1290 Ubiquitination
S1312 Phosphorylation

研究背景

功能:

Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.

翻译修饰:

Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding.

Phosphorylated in response to DNA damage by ATM and/or ATR.

细胞定位:

Nucleus.
Note: Observed in spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites. They are frequently interlinked through BLM-associated ultra-fine DNA bridges.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with FANCD2; the interaction is direct. Interacts with FANCL. Interacts with MTMR15/FAN1. Interacts with POLN.

蛋白家族:

The C-terminal 30 residues are probably required for function in DNA repair.

研究领域

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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