产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
展开/折叠
GDB; Golli MBP; Golli MBP; myelin basic protein; Hemopoietic MBP; HMBPR; HUGO; MBP; MBP_CAVPO; MBP_HUMAN; MGC99675; MLD; Myelin A1 protein; Myelin A1 Protein, basic; Myelin basic protein; Myelin Deficient; Myelin membrane encephalitogenic protein; OTTHUMP00000163776; OTTHUMP00000174387; OTTHUMP00000174388; SHI; Shiverer; SP;
抗原和靶标
A synthesized peptide derived from human MBP.
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
- P02686 MBP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGNHAGKRELNAEKASTNSETNRGESEKKRNLGELSRTTSEDNEVFGEADANQNNGTSSQDTAVTDSKRTADPKNAWQDAHPADPGSRPHLIRLFSRDAPGREDNTFKDRPSESDELQTIQEDSAATSESLDVMASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARR
翻译修饰 - P02686 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S16 | Phosphorylation | Uniprot | |
S19 | Phosphorylation | Uniprot | |
S26 | Phosphorylation | Uniprot | |
S36 | Phosphorylation | Uniprot | |
T38 | Phosphorylation | Uniprot | |
T39 | Phosphorylation | Uniprot | |
S40 | Phosphorylation | Uniprot | |
T65 | Phosphorylation | Uniprot | |
S87 | Phosphorylation | Uniprot | |
S96 | Phosphorylation | P27361 (MAPK3) | Uniprot |
T106 | Phosphorylation | Uniprot | |
S114 | Phosphorylation | Uniprot | |
S141 | Phosphorylation | Uniprot | |
S146 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y148 | Phosphorylation | Uniprot | |
S153 | Phosphorylation | Uniprot | |
T154 | Phosphorylation | Uniprot | |
T169 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S190 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y203 | Phosphorylation | Uniprot | |
S205 | Phosphorylation | Uniprot | |
T229 | Phosphorylation | P28482 (MAPK1) | Uniprot |
T232 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
R241 | Methylation | Uniprot | |
S249 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y261 | Phosphorylation | Uniprot | |
S266 | Phosphorylation | P17612 (PRKACA) | Uniprot |
T283 | Phosphorylation | Uniprot | |
S285 | Phosphorylation | Uniprot | |
S295 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S299 | Phosphorylation | Q8TAS1 (UHMK1) | Uniprot |
研究背景
The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.
Plasma membrane>Myelin membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Cytoplasmic side of myelin.
Nucleus.
Note: Targeted to nucleus in oligodendrocytes.
MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Homodimer. Isoform 3 exists as a homodimer.
Belongs to the myelin basic protein family.
文献引用
Application: WB Species: Human Sample: HEK293T cells
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