产品: CD79A 小鼠 单克隆 抗体
货号: BF8329
描述: Mouse monoclonal antibody to CD79A
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Dog
分子量: 25KD; 25kD(Calculated).
蛋白号: P11912

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Monoclonal [AFfirm8329]
特异性:
CD79A Mouse Monoclonal Antibody detects endogenous levels of total CD79A
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

B lymphocyte-specific MB1 protein; B-cell antigen receptor complex-associated protein alpha chain; CD 79a; CD79a; CD79a antigen (immunoglobulin-associated alpha); CD79A antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A_HUMAN; Ig alpha; Ig-alpha; IGA; IgM-alpha; Immunoglobulin-associated alpha; Ly54; MB-1 membrane glycoprotein; MB1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
序列:
MPGGPGVLQALPATIFLLFLLSAVYLGPGCQALWMHKVPASLMVSLGEDAHFQCPHNSSNNANVTWWRVLHGNYTWPPEFLGPGEDPNGTLIIQNVNKSHGGIYVCRVQEGNESYQQSCGTYLRVRQPPPRPFLDMGEGTKNRIITAEGIILLFCAVVPGTLLLFRKRWQNEKLGLDAGDEYEDENLYEGLNLDDCSMYEDISRGLQGTYQDVGSLNIGDVQLEKP

翻译修饰 - P11912 作为底物

Site PTM Type Enzyme
T140 Phosphorylation
K141 Ubiquitination
T161 Phosphorylation
K173 Ubiquitination
Y182 Phosphorylation
Y188 Phosphorylation P51451 (BLK) , P07948 (LYN)
S197 Phosphorylation P43405 (SYK)
Y199 Phosphorylation P51451 (BLK) , P06241 (FYN)
S203 Phosphorylation P43405 (SYK)
T209 Phosphorylation
Y210 Phosphorylation
S215 Phosphorylation
K225 Ubiquitination

研究背景

功能:

Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.

翻译修饰:

Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylated by LYN. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.

Arginine methylation in the ITAM domain may interfere with the binding of SYK. It promotes signals leading to B-cell differentiation (By similarity).

细胞定位:

Cell membrane>Single-pass type I membrane protein.
Note: Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

B-cells.

亚基结构:

Heterodimer of alpha and beta chains; disulfide-linked. Part of the B-cell antigen receptor complex where the alpha/beta chain heterodimer is non-covalently associated with an antigen-specific membrane-bound surface immunoglobulin of two heavy chains and two light chains. Interacts through its phosphorylated ITAM domain with the SH2 domains of SYK which stimulates SYK autophosphorylation and activation. Also interacts, when phosphorylated on Tyr-210, with the SH2 domain of BLNK/SLP65, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK which is necessary for trafficking of the BCR to late endosomes. Interacts with Src-family tyrosine kinases including FYN and LYN, increasing their activity (By similarity).

研究领域

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

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