WB, IHC, IF/ICC, ELISA

Hm, Ms, Rt, Fs, Mk, Rb

货号	产品	规格	来源	应用	反应	文献引用
AF6308	mTOR Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt,Fs	104
AF3308	P-mTOR (Ser2448) Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt,Fs	124
AF3309	P-mTOR (Ser2481) Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	7
DF7527	Raptor Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	4
AF2697	Rictor Ab	20ul	Rabbit	WB	Hm,Ms,Rt,Mk	1
DF6204	MLST8 Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt,Mk	1
AF7011	Tubulin beta Ab	20ul	Rabbit	WB,IHC,IF/ICC	Hm,Ms,Rt	99
S0001	Goat Anti-Rabbit IgG HRP	100ul	Goat	WB,IHC,ELISA	Rb	579

The mammalian target of rapamycin (mTOR) pathway is a central signaling pathway that regulates various cellular processes, including cell growth, proliferation, metabolism, and autophagy, in response to changes in nutrient availability, energy status, and growth factor signaling.

Store at -20 °C. Stable for 12 months from date of receipt.

The mammalian target of rapamycin (mTOR) pathway is a central signaling pathway that regulates various cellular processes, including cell growth, proliferation, metabolism, and autophagy, in response to changes in nutrient availability, energy status, and growth factor signaling.

The mTOR protein kinase exists in two distinct complexes: mTOR Complex 1 (mTORC1) and mTOR Complex 2 (mTORC2). Each complex has unique protein components and substrates, and they regulate different aspects of cellular physiology.

1. **mTORC1:** mTORC1 is activated in response to growth factors, amino acids, and cellular energy status. It regulates protein synthesis, cell growth, and metabolism by phosphorylating downstream targets such as ribosomal protein S6 kinase 1 (S6K1) and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), which control mRNA translation and protein synthesis. mTORC1 also inhibits autophagy by phosphorylating and inactivating ULK1 (Unc-51-like autophagy activating kinase 1) and other autophagy-related proteins.

2. **mTORC2:** mTORC2 is less well-characterized than mTORC1 but is known to regulate cell survival, cytoskeletal organization, and metabolism. It phosphorylates and activates protein kinase B (Akt) and other members of the AGC kinase family, which play key roles in cell growth, survival, and metabolism.

Key proteins and regulators involved in the mTOR pathway include:

1. **Rheb (Ras homolog enriched in brain):** Rheb is a small GTPase that directly activates mTORC1 in response to growth factor signaling and nutrient availability.

2. **TSC1-TSC2 complex:** The TSC1-TSC2 complex serves as a key negative regulator of mTORC1 by acting as a GTPase-activating protein (GAP) for Rheb, thereby inhibiting its ability to activate mTORC1.

3. **PI3K/Akt signaling pathway:** Growth factor signaling through the PI3K/Akt pathway leads to the activation of mTORC1 via phosphorylation and inhibition of the TSC1-TSC2 complex, promoting cell growth and proliferation.

4. **AMP-activated protein kinase (AMPK):** AMPK is a cellular energy sensor that negatively regulates mTORC1 activity under conditions of low energy availability by phosphorylating and activating the TSC1-TSC2 complex.

The mTOR pathway plays a central role in coordinating cellular responses to changes in nutrient and energy status, growth factor signaling, and cellular stress. Dysregulation of the mTOR pathway has been implicated in various diseases, including cancer, metabolic disorders, neurodegenerative diseases, and aging. Therefore, understanding the molecular mechanisms underlying mTOR signaling and the roles of key proteins in this pathway is essential for elucidating disease pathogenesis and developing therapeutic strategies targeting the mTOR pathway for the treatment of these disorders.