产品: LIMK1/2 小鼠 单克隆 抗体
货号: BF8933
描述: Mouse monoclonal antibody to LIMK1/2
应用: WB
反应: Human
预测: Mouse, Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 72kDa; 73kD,72kD(Calculated).
蛋白号: P53667 | P53671

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产品描述

来源:
Mouse
应用:
WB 1:500-1:3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Monoclonal [AFfirm8933]
特异性:
LIMK1/2 Mouse Monoclonal Antibody detects endogenous levels of total LIMK1/2.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 2.7.1.37; LIM domain containing protein kinase; LIM domain kinase 1; LIM kinase; LIM motif containing protein kinase; LIMK 1; LIMK; LIMK-1; limk1; LIMK1_HUMAN; LIM containing protein kinase 2b; LIM domain kinase 2; LIM domain kinase 2b; LIM kinase 2b; LIM motif containing protein kinase 2b; LIMK 2b; LIMK-2; Limk2; LIMK2 b; LIMK2_HUMAN; LIMK2b;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P53667 LIMK1_HUMAN:

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

P53671 LIMK2_HUMAN:

Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

描述:
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified.
序列:
MRLTLLCCTWREERMGEEGSELPVCASCGQRIYDGQYLQALNADWHADCFRCCDCSASLSHQYYEKDGQLFCKKDYWARYGESCHGCSEQITKGLVMVAGELKYHPECFICLTCGTFIGDGDTYTLVEHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHVGDRILEINGTPIRNVPLDEIDLLIQETSRLLQLTLEHDPHDTLGHGLGPETSPLSSPAYTPSGEAGSSARQKPVLRSCSIDRSPGAGSLGSPASQRKDLGRSESLRVVCRPHRIFRPSDLIHGEVLGKGCFGQAIKVTHRETGEVMVMKELIRFDEETQRTFLKEVKVMRCLEHPNVLKFIGVLYKDKRLNFITEYIKGGTLRGIIKSMDSQYPWSQRVSFAKDIASGMAYLHSMNIIHRDLNSHNCLVRENKNVVVADFGLARLMVDEKTQPEGLRSLKKPDRKKRYTVVGNPYWMAPEMINGRSYDEKVDVFSFGIVLCEIIGRVNADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

翻译修饰 - P53667/P53671 作为底物

Site PTM Type Enzyme
S58 Phosphorylation
S210 Phosphorylation
K214 Ubiquitination
T229 Phosphorylation
T253 Phosphorylation
T270 Phosphorylation
S274 Phosphorylation
S286 Phosphorylation
S287 Phosphorylation
S296 Phosphorylation
S298 Phosphorylation
S302 Phosphorylation
S307 Phosphorylation O14965 (AURKA)
S310 Phosphorylation Q16539 (MAPK14)
S313 Phosphorylation
R320 Methylation
S323 Phosphorylation P49137 (MAPKAPK2)
S337 Phosphorylation
K368 Ubiquitination
T508 Phosphorylation Q9NQU5 (PAK6) , Q13464 (ROCK1) , Q13153 (PAK1) , Q16566 (CAMK4) , O14965 (AURKA)
S534 Phosphorylation
S637 Phosphorylation
Site PTM Type Enzyme
K57 Ubiquitination
K62 Ubiquitination
S149 Phosphorylation
T151 Phosphorylation
S166 Phosphorylation
S168 Phosphorylation
T179 Phosphorylation
S191 Phosphorylation
T210 Phosphorylation
K272 Ubiquitination
T278 Phosphorylation
S283 Phosphorylation O14965 (AURKA) , Q05655 (PRKCD)
S287 Phosphorylation
S289 Phosphorylation
S291 Phosphorylation
K292 Ubiquitination
S293 Phosphorylation
S297 Phosphorylation
S298 Phosphorylation
K300 Ubiquitination
S310 Phosphorylation
S318 Phosphorylation
K347 Ubiquitination
K355 Acetylation
K355 Ubiquitination
K360 Ubiquitination
K371 Ubiquitination
K378 Ubiquitination
K414 Ubiquitination
K428 Ubiquitination
K487 Ubiquitination
T494 Phosphorylation O14965 (AURKA)
T505 Phosphorylation Q5VT25 (CDC42BPA) , O14965 (AURKA) , Q13464 (ROCK1) , O75116 (ROCK2)

翻译修饰 - P53667/P53671 作为激酶

Substrate Site Source
P23528 (CFL1) S3 Uniprot
P50281 (MMP14) Y573 Uniprot
P53667-1 (LIMK1) S298 Uniprot
P53667-1 (LIMK1) S310 Uniprot
P60981 (DSTN) S3 Uniprot
Q9Y281-1 (CFL2) S3 Uniprot
Substrate Site Source
P23528 (CFL1) S3 Uniprot
P60981 (DSTN) S3 Uniprot

研究背景

功能:

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development.

Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

翻译修饰:

Autophosphorylated. Phosphorylated on Thr-508 by ROCK1 and PAK1, resulting in activation. Phosphorylated by PAK4 which increases the ability of LIMK1 to phosphorylate cofilin. Phosphorylated at Ser-323 by MAPKAPK2 during activation of VEGFA-induced signaling, which results in activation of LIMK1 and promotion of actin reorganization, cell migration, and tubule formation of endothelial cells. Dephosphorylated and inactivated by SSH1. Phosphorylated by CDC42BP.

Ubiquitinated. 'Lys-48'-linked polyubiquitination by RNF6 leads to proteasomal degradation through the 26S proteasome, modulating LIMK1 levels in the growth cone and its effect on axonal outgrowth. Also polyubiquitinated by RLIM (By similarity).

细胞定位:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium.
Note: Predominantly found in the cytoplasm. Localizes in the lamellipodium in a CDC42BPA, CDC42BPB and FAM89B/LRAP25-dependent manner.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

亚基结构:

Interacts (via LIM domain) with the cytoplasmic domain of NRG1. Interacts with NISCH. Interacts with RLIM and RNF6 (By similarity). Self-associates to form homodimers. Interacts with HSP90AA1; this interaction promotes LIMK1 dimerization and subsequent transphosphorylation. Interacts with CDKN1C. Interacts with SSH1. Interacts with ROCK1. Interacts (via LIM zinc-binding domains) with FAM89B/LRAP25 (via LRR repeat). Forms a tripartite complex with CDC42BPA, CDC42BPB and FAM89B/LRAP25 (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

功能:

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

翻译修饰:

Phosphorylated on serine and/or threonine residues by ROCK1.

细胞定位:

Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.

Cytoplasm. Nucleus.
Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus.

Cytoplasm. Nucleus.
Note: Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

亚基结构:

Binds ROCK1 and MARF1 (Ref.9,. Interacts with NISCH (By similarity).

蛋白家族:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

研究领域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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