HOXA1 Antibody - #DF3187

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产品: HOXA1 抗体
货号: DF3187
描述: Rabbit polyclonal antibody to HOXA1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 37 KD; 37kD(Calculated).
蛋白号: P49639
RRID: AB_2835410

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(100%)
克隆:
Polyclonal
特异性:
HOXA1 Antibody detects endogenous levels of total HOXA1.
RRID:
AB_2835410
引用格式: Affinity Biosciences Cat# DF3187, RRID:AB_2835410.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BSAS; Homeo box A1; Homeobox 1F; Homeobox A1; Homeobox protein Hox A1; Homeobox protein Hox-1F; Homeobox protein Hox-A1; Hox 1.6 like protein; Hox 1F; HOX A1; HOX A1 homeodomain protein; HOX1; HOX1F; hoxa1; hoxb1b; HXA1_HUMAN; Lab like protein; MGC45232;

抗原和靶标

免疫原:
Uniprot:

P49639(HXA1_HUMAN) >>Visit HPA database.

基因/基因ID:
HOXA1(3198)
序列:
MDNARMNSFLEYPILSSGDSGTCSARAYPSDHRITTFQSCAVSANSCGGDDRFLVGRGVQIGSPHHHHHHHHHHPQPATYQTSGNLGVSYSHSSCGPSYGSQNFSAPYSPYALNQEADVSGGYPQCAPAVYSGNLSSPMVQHHHHHQGYAGGAVGSPQYIHHSYGQEHQSLALATYNNSLSPLHASHQEACRSPASETSSPAQTFDWMKVKRNPPKTGKVGEYGYLGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQKKREKEGLLPISPATPPGNDEKAEESSEKSSSSPCVPSPGSSTSDTLTTSH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P49639 作为底物

Site PTM Type Enzyme
Y28 Phosphorylation
S30 Phosphorylation
T255 Phosphorylation
S296 Phosphorylation
T299 Phosphorylation
S334 Phosphorylation

研究背景

功能:

Sequence-specific transcription factor (By similarity). Regulates multiple developmental processes including brainstem, inner and outer ear, abducens nerve and cardiovascular development and morphogenesis as well as cognition and behavior. Also part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments (By similarity). Activates transcription in the presence of PBX1A and PKNOX1 (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with OGT (via TPR repeats domain); the interaction takes place mainly in the nucleus.

蛋白家族:

Belongs to the Antp homeobox family. Labial subfamily.

文献引用

1). Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway. eBioMedicine, 2022 (PubMed: 35525203) [IF=11.1]
2). HOXA1 participates in VSMC-to-macrophage-like cell transformation via regulation of NF-κB p65 and KLF4: a potential mechanism of atherosclerosis pathogenesis. Molecular Medicine, 2023 (PubMed: 37528397) [IF=5.7]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 1 HOXA1 expression is increased in the aortic root of AS mice. A Oil red-O staining for the aortic root of wild-type mice with ND, wild-type mice with HFD, or ApoE−/− mice with HFD. Scale bar = 500 μm (40 ×). B Immunofluorescence double staining for HOXA1 (red) and α-SMA (green) in each group. DAPI (blue) was used for nucleus staining. Scale bar = 50 μm (400 ×). C Western blot for HOXA1 expression in aortic root of wild-type or HFD-fed ApoE−/− mice. Data are expressed as mean ± SD (6 animals/group). Ordinary one-way ANOVA followed by Tukey's multiple comparison test was used to calculate the P value in panel C ***P 

Application: WB    Species: Mouse    Sample:

Fig. 1 HOXA1 expression is increased in the aortic root of AS mice. A Oil red-O staining for the aortic root of wild-type mice with ND, wild-type mice with HFD, or ApoE−/− mice with HFD. Scale bar = 500 μm (40 ×). B Immunofluorescence double staining for HOXA1 (red) and α-SMA (green) in each group. DAPI (blue) was used for nucleus staining. Scale bar = 50 μm (400 ×). C Western blot for HOXA1 expression in aortic root of wild-type or HFD-fed ApoE−/− mice. Data are expressed as mean ± SD (6 animals/group). Ordinary one-way ANOVA followed by Tukey's multiple comparison test was used to calculate the P value in panel C

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