产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0481, RRID:AB_2833503.
展开/折叠
ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; SOX 2; Sox2; SOX2_HUMAN; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; Transcription factor SOX 2; Transcription factor SOX-2; ysb;
抗原和靶标
Purified recombinant fragment of human SOX2 expressed in E. Coli.
- P48431 SOX2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM
翻译修饰 - P48431 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S37 | Phosphorylation | Uniprot | |
| K73 | Acetylation | Uniprot | |
| T116 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K121 | Ubiquitination | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| K245 | Sumoylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot |
研究背景
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Nucleus.
Interacts with ZSCAN10 (By similarity). Interacts with SOX3 and FGFR1 (By similarity). Interacts with GLIS1. Interacts with POU5F1; binds synergistically with POU5F1 to DNA (By similarity).
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
限制条款
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