| 产品: | RIN1 抗体 |
| 货号: | DF4384 |
| 描述: | Rabbit polyclonal antibody to RIN1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Horse, Dog |
| 分子量: | 84 KD; 84kD(Calculated). |
| 蛋白号: | Q13671 |
| RRID: | AB_2836739 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF4384, RRID:AB_2836739.
展开/折叠
Ras and Rab interactor 1; ras inhibitor 1; ras inhibitor; Ras inhibitor JC99; ras inhibitor RIN1; Ras interaction/interference protein 1; RIN 1;
抗原和靶标
Expressed in all tissues examined with high levels in brain, placenta and pancreas.
- Q13671 RIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESPGESGAGSPGAPSPSSFTTGHLAREKPAQDPLYDVPNASGGQAGGPQRPGRVVSLRERLLLTRPVWLQLQANAAAALHMLRTEPPGTFLVRKSNTRQCQALCMRLPEASGPSFVSSHYILESPGGVSLEGSELMFPDLVQLICAYCHTRDILLLPLQLPRAIHHAATHKELEAISHLGIEFWSSSLNIKAQRGPAGGPVLPQLKARSPQELDQGTGAALCFFNPLFPGDLGPTKREKFKRSFKVRVSTETSSPLSPPAVPPPPVPVLPGAVPSQTERLPPCQLLRRESSVGYRVPAGSGPSLPPMPSLQEVDCGSPSSSEEEGVPGSRGSPATSPHLGRRRPLLRSMSAAFCSLLAPERQVGRAAAALMQDRHTAAGQLVQDLLTQVRAGPEPQELQGIRQALSRARAMLSAELGPEKLLSPKRLEHVLEKSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13671 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| Y36 | Phosphorylation | P42684 (ABL2) | Uniprot |
| S42 | Phosphorylation | Uniprot | |
| T65 | Phosphorylation | Uniprot | |
| T90 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S258 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Q9BZL6 (PRKD2) , Q15139 (PRKD1) | Uniprot |
| Y295 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | P98161 (PKD1) , Q15139 (PRKD1) | Uniprot |
| T377 | Phosphorylation | Uniprot | |
| T388 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| Y632 | Phosphorylation | Uniprot | |
| R664 | Methylation | Uniprot | |
| Y676 | Phosphorylation | Uniprot | |
| Y681 | Phosphorylation | Uniprot | |
| R692 | Methylation | Uniprot | |
| T696 | Phosphorylation | Uniprot | |
| Y698 | Phosphorylation | Uniprot | |
| Y701 | Phosphorylation | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| S744 | Phosphorylation | Uniprot |
研究背景
Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.
Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylation at Ser-351 by PRKD1 induces interaction with 14-3-3 proteins.
Cytoplasm. Membrane. Cytoplasm>Cytoskeleton.
Note: Some amount is membrane-associated.
Expressed in all tissues examined with high levels in brain, placenta and pancreas.
Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351.
Belongs to the RIN (Ras interaction/interference) family.
研究领域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
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