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Figure 1: Western blot analysis using NCOA3 mouse mAb against truncated Trx-NCOA3 recombinant protein (1) and truncated NCOA3 (aa1-200)-hIgGFc transfected CHOK1 cell lysates (2).

产品:	SRC3 抗体
货号:	BF0072
描述:	Mouse monoclonal antibody to SRC3
应用:	WB ELISA
反应:	Human
分子量:	155kDa; 155kD(Calculated).
蛋白号:	Q9Y6Q9
RRID:	AB_2833670

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规格	价格	库存
50ul	RMB¥ 1800	现货
100ul	RMB¥ 2800	现货

货期: 当天发货

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实验方案

产品描述

来源:

Mouse

应用:

ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human

克隆:

Monoclonal [AFB1646]

特异性:

SRC3 antibody detects endogenous levels of total SRC3.

RRID:

AB_2833670
引用格式: Affinity Biosciences Cat# BF0072, RRID:AB_2833670.

偶联:

Unconjugated.

纯化:

Affinity-chromatography.

保存:

Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

ACTR; AIB 1; AIB-1; AIB1; Amplified in breast cancer 1; Amplified in breast cancer 1 protein; bHLHe42; CAGH 16; CAGH16; CBP interacting protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG 26; CTG26; KAT13B / AIB1; MGC141848; NCOA 3; NCoA-3; Ncoa3; NCOA3_HUMAN; Nuclear receptor coactivator 3; pCIP; RAC 3; RAC-3; RAC3; Receptor associated coactivator 3; Receptor-associated coactivator 3; SRC 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM 1; TRAM-1; TRAM1;

抗原和靶标

免疫原:

Purified recombinant fragment of human SRC3 expressed in E. Coli.

Uniprot:

Q9Y6Q9(NCOA3_HUMAN) >>Visit HPA database.

基因/基因ID:

NCOA3(8202)

表达:

Q9Y6Q9 NCOA3_HUMAN:

Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

描述:

NCOA3: nuclear receptor coactivator 3. The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation.

序列:

Q9Y6Q9 NCOA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC

翻译修饰 - Q9Y6Q9 作为底物

Q9Y6Q9

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
T24	Phosphorylation	P45983 (MAPK8) , Q16539 (MAPK14)	Uniprot
T30	Phosphorylation		Uniprot
S32	Phosphorylation		Uniprot
K35	Acetylation		Uniprot
K65	Ubiquitination		Uniprot
K97	Ubiquitination		Uniprot
S101	Phosphorylation		Uniprot
S102	Phosphorylation	P49674 (CSNK1E)	Uniprot
K167	Ubiquitination		Uniprot
T184	Phosphorylation		Uniprot
K191	Ubiquitination		Uniprot
S214	Phosphorylation		Uniprot
S244	Phosphorylation		Uniprot
R251	Methylation		Uniprot
S274	Phosphorylation		Uniprot
K276	Acetylation		Uniprot
T282	Phosphorylation		Uniprot
S284	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
S288	Phosphorylation		Uniprot
S306	Phosphorylation		Uniprot
S505	Phosphorylation	P45983 (MAPK8) , Q16539 (MAPK14)	Uniprot
S509	Phosphorylation		Uniprot
S543	Phosphorylation	P45983 (MAPK8) , Q16539 (MAPK14)	Uniprot
S551	Phosphorylation		Uniprot
T557	Phosphorylation		Uniprot
S563	Phosphorylation		Uniprot
S569	Phosphorylation		Uniprot
Y574	Phosphorylation		Uniprot
S601	Phosphorylation	P48730 (CSNK1D)	Uniprot
K616	Acetylation		Uniprot
K619	Acetylation		Uniprot
K620	Acetylation		Uniprot
K620	Ubiquitination		Uniprot
S636	Phosphorylation		Uniprot
S640	Phosphorylation		Uniprot
S664	Phosphorylation		Uniprot
K687	Acetylation		Uniprot
S694	Phosphorylation		Uniprot
T714	Phosphorylation		Uniprot
S715	Phosphorylation		Uniprot
K723	Sumoylation		Uniprot
S728	Phosphorylation	P06493 (CDK1)	Uniprot
K753	Ubiquitination		Uniprot
K786	Sumoylation		Uniprot
S794	Phosphorylation		Uniprot
S836	Phosphorylation		Uniprot
K840	Methylation		Uniprot
R849	Methylation		Uniprot
S857	Phosphorylation	O15111 (CHUK) , Q16659 (MAPK6) , P17612 (PRKACA)	Uniprot
S860	Phosphorylation	Q16539 (MAPK14) , P45983 (MAPK8)	Uniprot
S863	Phosphorylation		Uniprot
S866	Phosphorylation		Uniprot
S867	Phosphorylation	P06493 (CDK1) , Q16539 (MAPK14) , P45983 (MAPK8)	Uniprot
K926	Ubiquitination		Uniprot
R951	Methylation		Uniprot
S1033	Phosphorylation	Q05513 (PRKCZ)	Uniprot
S1042	Phosphorylation	Q05513 (PRKCZ)	Uniprot
S1048	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T1059	Phosphorylation	Q05513 (PRKCZ)	Uniprot
S1062	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T1064	Phosphorylation	Q05513 (PRKCZ)	Uniprot
T1067	Phosphorylation	Q05513 (PRKCZ)	Uniprot
K1091	Methylation		Uniprot
T1114	Phosphorylation	Q05513 (PRKCZ)	Uniprot
R1171	Methylation		Uniprot
R1177	Methylation		Uniprot
R1188	Methylation		Uniprot
K1194	Sumoylation		Uniprot
S1330	Phosphorylation		Uniprot
Y1357	Phosphorylation	P00519 (ABL1)	Uniprot

研究背景

Q9Y6Q9_NCOA3_HUMAN

功能:

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.

翻译修饰:

Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.

Methylated by CARM1.

Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.

细胞定位:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

组织特异性:

Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

亚基结构:

Interacts with CARM1 (By similarity). Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP. Interacts with CASP8AP2, NR3C1 and PCAF. Interacts with ATAD2 and this interaction is enhanced by estradiol. Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with PSMB9. Interacts with NPAS2. Interacts with NR4A3 (By similarity). Interacts with ESRRB; mediates the interaction between ESRRB and RNA polymerase II complexes and allows NCOA3 corecruitment to ESRRB, KLF4, NANOG, and SOX2 enhancer regions to trigger ESRRB-dependent gene activation involved in self-renewal and pluripotency (By similarity).

蛋白家族:

Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

Belongs to the SRC/p160 nuclear receptor coactivator family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

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SRC3 Antibody - #BF0072

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