| 产品: | LPL 抗体 |
| 货号: | BF0636 |
| 描述: | Mouse monoclonal antibody to LPL |
| 应用: | WB ELISA |
| 反应: | Human |
| 分子量: | 53.1kDa; 53kD(Calculated). |
| 蛋白号: | P06858 |
| RRID: | AB_2833701 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0636, RRID:AB_2833701.
展开/折叠
EC 3.1.1; EC 3.1.1.34; HDLCQ11; LIPD; LIPL_HUMAN; Lipoprotein lipase; LPL; LPL protein; MGC137861;
抗原和靶标
Purified recombinant fragment of human LPL expressed in E. Coli.
Detected in blood plasma (PubMed:2340307, PubMed:11893776, PubMed:12641539). Detected in milk (at protein level) (PubMed:2340307).
- P06858 LIPL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESKALLVLTLAVWLQSLTASRGGVAAADQRRDFIDIESKFALRTPEDTAEDTCHLIPGVAESVATCHFNHSSKTFMVIHGWTVTGMYESWVPKLVAALYKREPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSLGAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPGRSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERSIHLFIDSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEINKVRAKRSSKMYLKTRSQMPYKVFHYQVKIHFSGTESETHTNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFLIYTEVDIGELLMLKLKWKSDSYFSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKVSHLQKGKAPAVFVKCHDKSLNKKSG
翻译修饰 - P06858 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| T19 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| N70 | N-Glycosylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot | |
| K265 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| Y329 | Phosphorylation | Uniprot | |
| Y343 | Phosphorylation | Uniprot | |
| N386 | N-Glycosylation | Uniprot | |
| K408 | Acetylation | Uniprot | |
| T438 | Phosphorylation | Uniprot |
研究背景
Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans.
Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity.
Cell membrane>Peripheral membrane protein>Extracellular side. Secreted. Secreted>Extracellular space>Extracellular matrix.
Note: Newly synthesized LPL binds to cell surface heparan proteoglycans and is then released by heparanase. Subsequently, it becomes attached to heparan proteoglycan on endothelial cells (PubMed:27811232). Locates to the plasma membrane of microvilli of hepatocytes with triglyceride-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity).
Detected in blood plasma. Detected in milk (at protein level).
Homodimer (Probable). Interacts with GPIHBP1 with 1:1 stoichiometry. Interacts with APOC2; the interaction activates LPL activity in the presence of lipids (By similarity). Interaction with heparan sulfate proteoglycans is required to protect LPL against loss of activity. Associates with lipoprotein particles in blood plasma. Interacts with LMF1 and SEL1L; interaction with SEL1L is required to prevent aggregation of newly synthesized LPL in the endoplasmic reticulum (ER), and for normal export of LPL from the ER to the extracellular space. Interacts with SORL1; SORL1 acts as a sorting receptor, promoting LPL localization to endosomes and later to lysosomes, leading to degradation of newly synthesized LPL.
Belongs to the AB hydrolase superfamily. Lipase family.
研究领域
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Metabolism > Lipid metabolism > Glycerolipid metabolism.
· Organismal Systems > Endocrine system > PPAR signaling pathway.
· Organismal Systems > Digestive system > Cholesterol metabolism.
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