TOMM20 Antibody - #AF5206

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产品: TOMM20 抗体
货号: AF5206
描述: Rabbit polyclonal antibody to TOMM20
应用: WB IHC IF/ICC
文献应用: WB
反应: Human, Mouse, Rat
文献反应: Rat, Mouse
预测: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
分子量: 16 kDa; 16kD(Calculated).
蛋白号: Q15388
RRID: AB_2837692

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(82%)
克隆:
Polyclonal
特异性:
TOMM20 Antibody detects endogenous levels of total TOMM20.
RRID:
AB_2837692
引用格式: Affinity Biosciences Cat# AF5206, RRID:AB_2837692.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM20; TOM20_HUMAN; TOMM20; Translocase of outer mitochondrial membrane 20 homolog (yeast); Translocase of outer mitochondrial membrane 20 homolog type II;

抗原和靶标

免疫原:
Uniprot:

Q15388(TOM20_HUMAN) >>Visit HPA database.

基因/基因ID:
TOMM20(9804)
描述:
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore
序列:
MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
82
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15388 作为底物

Site PTM Type Enzyme
Ubiquitination
K35 Ubiquitination
S55 Phosphorylation
K56 Acetylation
K56 Ubiquitination
K61 Ubiquitination
K68 Ubiquitination
S135 Phosphorylation
S138 Phosphorylation

研究背景

功能:

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.

翻译修饰:

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

细胞定位:

Mitochondrion outer membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22. Interacts with APEX1.

蛋白家族:

Belongs to the Tom20 family.

文献引用

1). The role of the apoptosis-related protein BCL-B in the regulation of mitophagy in hepatic stellate cells during the regression of liver fibrosis. EXPERIMENTAL AND MOLECULAR MEDICINE, 2019 (PubMed: 30635551) [IF=9.5]

Application: WB    Species: mouse    Sample: hepatic fibrosis

Fig. 4 |Inhibition of mitophagy aggravates hepatic fibrosis in mice. a H&E staining of liver sections from mice; n = 6 per group; scale bar, 50 μm.b Sirius red staining of collagen in mouse liver sections; n = 6 per group; scale bar, 50 μm. c Representative western blots of TOM20, collagen I and α-SMA. Bar graph represents the mean ± SEM of three different experiments. *P < 0.05; **P < 0.01; and ***P < 0.001 vs. the indicated groups. d Serum levels of ALT and AST in the indicated mice. Bar graph represents the mean ± SEM; n = 6 per group. *P < 0.05 vs. The indicated groups
2). PBLD promotes IRF3 mediated the type I interferon (IFN-I) response and apoptosis to inhibit viral replication. Cell death & disease, 2024 (PubMed: 39362857) [IF=8.1]
3). PINK1/Parkin pathway-mediated mitophagy by AS-IV to explore the molecular mechanism of muscle cell damage. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 36948131) [IF=6.9]

Application: WB    Species: Rat    Sample: L6 myoblasts

Fig. 3. AS-IV ameliorates mitochondrial damage induced by H2O2 combined with CCCP in L6 myoblasts. (A, B) Effects of AS-IV on intracellular ROS (bar = 100 µm). (C) Effects of AS-IV on ATP activities. (D) Effects of AS-IV on Tom 20 mRNA expression. (E) Effects of AS-IV on Tim 23 mRNA expression. (F) Effects of AS-IV on VDAC1 mRNA expression. (G) Effects of AS-IV on Tom 20 protein expression. (H) Effects of AS-IV on Tim 23 protein expression. (I) Effects of AS-IV on VDAC1 protein expression. ★P 
4). Regulatory mechanism of perinatal nonylphenol exposure on cardiac mitochondrial autophagy and the PINK1/Parkin signaling pathway in male offspring rats. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38367424) [IF=6.7]
5). Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy. Free radical research, 2023 (PubMed: 37897414) [IF=3.6]
6). Mechanism of Baihu Renshen decoction on T2DM rats based on mitochondrial autophagy mediated by PINK1/Parkin. , 2023

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