PTEN Antibody - #BF0602

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产品: PTEN 抗体
货号: BF0602
描述: Mouse monoclonal antibody to PTEN
应用: WB IF/ICC ELISA FACS
文献验证: WB, IF/ICC
反应: Human, Mouse
蛋白号: P60484
RRID: AB_2833737

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   规格 价格 库存
 50ul RMB¥ 1800 现货
 100ul RMB¥ 2800 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Mouse
应用:
ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000, FCM 1:200-1:400
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Monoclonal [AFB1713]
特异性:
PTEN antibody detects endogenous levels of total PTEN.
RRID:
AB_2833737
引用格式: Affinity Biosciences Cat# BF0602, RRID:AB_2833737.
偶联:
Unconjugated.
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

10q23del; BZS; DEC; GLM2; MGC11227; MHAM; MMAC1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; Phosphatase and tensin like protein; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; Pten; PTEN_HUMAN; PTEN1; TEP1;

抗原和靶标

免疫原:

Purified recombinant fragment of human PTEN expressed in E. Coli.

基因/基因ID:
PTEN(5728)
描述:
PTEN (phosphatase and tensin homolog) was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. This protein is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.

文献引用

1). GFPT1 promotes the proliferation of cervical cancer via regulating the ubiquitination and degradation of PTEN. Carcinogenesis, 2022 (PubMed: 36040914) [IF=3.3]

Application: WB    Species: Human    Sample: Hela and Siha cells

Figure 3.GFPT1 promoted cervical cancer proliferation by regulating PTEN. (A) GSEA results showed the pathways that enriched in high GFPT1 expressed group. (B) PTEN expression in data downloaded from GEO database. (C, D, E) AKT, p-AKT and PTEN protein levels in Hela and Siha cells with GFPT1 knockdown or overexpression were detected by western blotting. (F, G) Co-immunoprecipitation of GFPT1 and PTEN in Hela and Siha cells. (H) Immunofluorescence results showed that GFPT1 co-localized with PTEN. ****, P 

Application: IF/ICC    Species: Human    Sample: Hela and Siha cells

Figure 3.GFPT1 promoted cervical cancer proliferation by regulating PTEN. (A) GSEA results showed the pathways that enriched in high GFPT1 expressed group. (B) PTEN expression in data downloaded from GEO database. (C, D, E) AKT, p-AKT and PTEN protein levels in Hela and Siha cells with GFPT1 knockdown or overexpression were detected by western blotting. (F, G) Co-immunoprecipitation of GFPT1 and PTEN in Hela and Siha cells. (H) Immunofluorescence results showed that GFPT1 co-localized with PTEN. ****, P 

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