产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# BF0632, RRID:AB_2833768.
展开/折叠
AGM8; bHLHb21; Class B basic helix-loop-helix protein 21; E12; E2A; E2A immunoglobulin enhancer binding factors E12/E47; E47; Helix loop helix protein HE47; Immunoglobulin enhancer-binding factor E12/E47; Immunoglobulin transcription factor 1; ITF1; Kappa-E2-binding factor; MGC129647; MGC129648; Negative vitamin D response element binding protein; NOL1-TCF3 fusion; TCF-3; Tcf3; TFE2_HUMAN; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor 3; transcription factor 3 variant 3; Transcription factor E2-alpha; Transcription factor ITF-1; VDIR; VDR interacting repressor; vitamin D receptor-interacting repressor;
抗原和靶标
Purified recombinant fragment of human TCF3 expressed in E. Coli.
- P15923 TFE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNQPQRMAPVGTDKELSDLLDFSMMFPLPVTNGKGRPASLAGAQFGGSGLEDRPSSGSWGSGDQSSSSFDPSRTFSEGTHFTESHSSLSSSTFLGPGLGGKSGERGAYASFGRDAGVGGLTQAGFLSGELALNSPGPLSPSGMKGTSQYYPSYSGSSRRRAADGSLDTQPKKVRKVPPGLPSSVYPPSSGEDYGRDATAYPSAKTPSSTYPAPFYVADGSLHPSAELWSPPGQAGFGPMLGGGSSPLPLPPGSGPVGSSGSSSTFGGLHQHERMGYQLHGAEVNGGLPSASSFSSAPGATYGGVSSHTPPVSGADSLLGSRGTTAGSSGDALGKALASIYSPDHSSNNFSSSPSTPVGSPQGLAGTSQWPRAGAPGALSPSYDGGLHGLQSKIEDHLDEAIHVLRSHAVGTAGDMHTLLPGHGALASGFTGPMSLGGRHAGLVGGSHPEDGLAGSTSLMHNHAALPSQPGTLPDLSRPPDSYSGLGRAGATAAASEIKREEKEDEENTSAADHSEEEKKELKAPRARTSPDEDEDDLLPPEQKAEREKERRVANNARERLRVRDINEAFKELGRMCQLHLNSEKPQTKLLILHQAVSVILNLEQQVRERNLNPKAACLKRREEEKVSGVVGDPQMVLSAPHPGLSEAHNPAGHM
翻译修饰 - P15923 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T12 | Phosphorylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| S39 | Phosphorylation | Q9P286 (PAK5) | Uniprot |
| S48 | Phosphorylation | Uniprot | |
| R105 | Methylation | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Q16539 (MAPK14) , P11802 (CDK4) , Q15759 (MAPK11) , P24941 (CDK2) | Uniprot |
| S141 | Phosphorylation | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| Y153 | Phosphorylation | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| K171 | Acetylation | Uniprot | |
| S245 | Phosphorylation | P11802 (CDK4) , P24941 (CDK2) | Uniprot |
| S341 | Phosphorylation | Q02779 (MAP3K10) | Uniprot |
| S345 | Phosphorylation | Uniprot | |
| S346 | Phosphorylation | Uniprot | |
| S350 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Q02779 (MAP3K10) | Uniprot |
| S354 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Q02779 (MAP3K10) | Uniprot |
| S359 | Phosphorylation | Q02779 (MAP3K10) | Uniprot |
| T366 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| R371 | Methylation | Uniprot | |
| S379 | Phosphorylation | Q02779 (MAP3K10) | Uniprot |
| S381 | Phosphorylation | Uniprot | |
| Y382 | Phosphorylation | Uniprot | |
| K392 | Sumoylation | Uniprot | |
| T411 | Phosphorylation | Uniprot | |
| K498 | Sumoylation | Uniprot | |
| K502 | Sumoylation | Uniprot | |
| S509 | Phosphorylation | Uniprot | |
| S514 | Phosphorylation | Uniprot | |
| T528 | Phosphorylation | Uniprot | |
| S529 | Phosphorylation | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| K614 | Ubiquitination | Uniprot | |
| K625 | Ubiquitination | Uniprot |
研究背景
Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.
Phosphorylated following NGF stimulation.
Nucleus.
Forms a heterodimer with ASH1 and TWIST2. Isoform E12 interacts with RALGAPA1 and FIGLA. Efficient DNA binding requires dimerization with another bHLH protein. Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3. Interacts with NEUROD2, PTF1A and TGFB1I1. Forms a heterodimer with MYOG; heterodimerization enhances MYOG DNA-binding and transcriptional activities (By similarity). Homodimer. Heterodimer. Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element. Interacts with EP300 and UBE2I. Interacts with BHLHA9. Forms a heterodimer with ATOH8; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters (By similarity).
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
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